Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SEC23IP / p125 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100985-100
Catalog Number: LS-G100985
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEC23IP (NM_007190), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- p125A | SEC23 interacting protein | SEC23-interacting protein | SEC23IP | p125 | MSTP053
- Origin Species
- Human
- NCBI GenBank
- NM_007190
- SwissProt
- Q9Y6Y8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 110.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAERKPNGGS GGASTSSSGT NLLFSSSATE FSFNVPFIPV TQASASPASL LLPGEDSTDV61GEEDSFLGQT SIHTSAPQTF SYFSQVSSSS DPFGNIGQSP LTTAATSVGQ SGFPKPLTAL121PFTTGSQDVS NAFSPSISKA QPGAPPSSLM GINSYLPSQP SSLPPSYFGN QPQGIPQPGY181NPYRHTPGSS RANPYIAPPQ LQQCQTPGPP AHPPPSGPPV QMYQMPPGSL PPVPSSVQSP241AQQQVPARPG APSVQVPSPF LLQNQYEPVQ PHWFYCKEVE YKQLWMPFSV FDSLNLEEIY301NSVQPDPESV VLGTDGGRYD VYLYDRIRKA AYWEEEPAEV RRCTWFYKGD TDSRFIPYTE361EFSEKLEAEY KKAVTTNQWH RRLEFPSGET IVMHNPKVIV QFQPSSVPDE WGTTQDGQTR421PRVVKRGIDD NLDEIPDGEM PQVDHLVFVV HGIGPVCDLR FRSIIECVDD FRVVSLKLLR481THFKKSLDDG KVSRVEFLPV HWHSSLGGDA TGVDRNIKKI TLPSIGRFRH FTNETLLDIL541FYNSPTYCQT IVEKVGMEIN HLHALFMSRN PDFKGGVSVA GHSLGSLILF DILSNQKDLN601LSKCPGPLAV ANGVVKQLHF QEKQMPEEPK LTLDESYDLV VENKEVLTLQ ETLEALSLSE661YFSTFEKEKI DMESLLMCTV DDLKEMGIPL GPRKKIANFV EHKAAKLKKA ASEKKAVAAT721STKGQEQSAQ KTKDMASLPS ESNEPKRKLP VGACVSSVCV NYESFEVGAG QVSVAYNSLD781FEPEIFFALG SPIAMFLTIR GVDRIDENYS LPTCKGFFNI YHPLDPVAYR LEPMIVPDLD841LKAVLIPHHK GRKRLHLELK ESLSRMGSDL KQGFISSLKS AWQTLNEFAR AHTSSTQLQE901ELEKVANQIK EEEEKQVVEA EKVVESPDFS KDEDYLGKVG MLNGGRRIDY VLQEKPIESF961NEYLFALQSH LCYWESEDTA LLLLKEIYRT MNISPEQPQHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1000 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.