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SEC14L5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SEC14L5 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SEC14L5 antibodies →
$725.00each
Catalog Number: LS-G113775-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SEC14L5 (NM_014692), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SEC14-like 5 (S. cerevisiae) | SEC14L5 | KIAA0420 | PRELID4B | SEC14-like protein 5
Origin Species
Human
NCBI GenBank
AB007880
SwissProt
O43304

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVQRYQSPVR VYKYPFELVM AAYEKRFPTC PQIPVFLGSE VLRESRSPDG AVHVVERSCR
61LRVDAPRLLR KIAGVEHVVF VQTNILNWKE RTLLIEAHNE TFANRVVVNE HCSYTVHPEN
121EDWTCFEQSA SLDIRSFFGF ENALEKIAMK QYTANVKRGK EVIEHYLNEL ISQGTSHIPR
181WTPAPVREED ARNQAGPRDP SSLEAHGPRS TLGPALEAVS MDGDKLDADY IERCLGHLTP
241MQESCLIQLR HWLQETHKGK IPKDEHILRF LRAHDFHLDK AREMLRQSLS WRKQHQVDLL
301LQTWQPPALL EEFYAGGWHY QDIDGRPLYI LRLGQMDTKG LMKAVGEEAL LRHVLSVNEE
361GQKRCEGSTR QLGRPISSWT CLLDLEGLNM RHLWRPGVKA LLRMIEVVED NYPETLGRLL
421IVRAPRVFPV LWTLISPFIN ENTRRKFLIY SGSNYQGPGG LVDYLDREVI PDFLGGESVC
481NVPEGGLVPK SLYMTEEEQE HTDQLWQWSE TYHSASVLRG APHEVAVEIL EGESVITWDF
541DILRGDVVFS LYHTKQAPRL GAREPGTRAS GQLIDKGWVL GRDYSRVEAP LVCREGESIQ
601GSHVTRWPGV YLLQWQMHSP PSSVACSLPG VDDVLTALHS PGPKCKLLYY CEVLASEDFR
661GSMSSLESCT SGFSQLSAAT SSSSSGQSHS SSLVSR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

696 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.