Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SDSL / Serine Dehydratase-Like Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107394-100
Catalog Number: LS-G107394
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SDSL (NM_138432), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SDSL | Serine dehydratase 2 | Serine dehydratase-like | SDH | TDH | L-serine deaminase | L-threonine dehydratase | SDH 2 | SDS-RS1
- Origin Species
- Human
- NCBI GenBank
- BF056112
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDGPVAEHAK QEPFHVVTPL LESWALSQVA GMPVFLKCEN VQPSGSFKIR GIGHFCQEMA61KKGCRHLVCS SGGNAGIAAA YAARKLGIPA TIVLPESTSL QVVQRLQGEG AEVQLTGKVW121DEANLRAQEL AKRDGWENVP PFDHPLIWKG HASLVQELKA VLRTPPGALV LAVGGGGLLA181GVVAGLLEVG WQHVPIIAME THGAHCFNAA ITAGKLVTLP DITSVAKSLG AKTVAARALE241CMQVCKIHSE VVEDTEAVSA VQQLLDDERM LVEPACGAAL AAIYSGLLRR LQAEGCLPPS301LTSVVVIVCG GNNINSRELQ ALKTHLGQVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
329 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.