LSBio
SDK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SDK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SDK2 antibodies →
$725.00each
Catalog Number: LS-G126435-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SDK2 (NM_001144952), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1514 | SDK2 | Sidekick homolog 2 | Sidekick homolog 2 (chicken) | Protein sidekick-2
Origin Species
Human
NCBI GenBank
NM_019064

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
239.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWGLLIWTLL ALHQIRAARA QDDVSPYFKT EPVRTQVHLE GNRLVLTCMA EGSWPLEFKW
61LHNNRELTKF SLEYRYMITS LDRTHAGFYR CIVRNRMGAL LQRQTEVQVA YMGSFEEGEK
121HQSVSHGEAA VIRAPRIASF PQPQVTWFRD GRKIPPSSRI AITLENTLVI LSTVAPDAGR
181YYVQAVNDKN GDNKTSQPIT LTVENVGGPA DPIAPTIIIP PKNTSVVAGT SEVTLECVAN
241ARPLIKLHII WKKDGVLLSG GISDHNRRLT IPNPTGSDAG YYECEAVLRS SSVPSVVRGA
301YLSVLEPPQF VKEPERHITA EMEKVVDIPC QAKGVPPPSI TWYKDAAVVE VEKLTRFRQR
361NDGGLQISGL VPDDTGMFQC FARNAAGEVQ TSTYLAVTSI APNITRGPLD STVIDGMSVV
421LACETSGAPR PAITWQKGER ILASGSVQLP RFTPLESGSL LISPTHISDA GTYTCLATNS
481RGVDEASADL VVWARTRITK PPQDQSVIKG TQASMVCGVT HDPRVTIRYI WEKDGATLGT
541ESHPRIRLDR NGSLHISQTW SGDIGTYTCR VISAGGNDSR SAHLRVRQLP HAPEHPVATL
601STVERRAINL TWTKPFDGNS PLIRYILEMS ENNAPWTVLL ASVDPKATSV TVKGLVPARS
661YQFRLCAVND VGKGQFSKDT ERVSLPEEPP TAPPQNVIAS GRTNQSIMIQ WQPPPESHQN
721GILKGYIIRY CLAGLPVGYQ FKNITDADVN NLLLEDLIIW TNYEIEVAAY NSAGLGVYSS
781KVTEWTLQGV PTVPPGNVHA EATNSTTIRF TWNAPSPQFI NGINQGYKLI AWEPEQEEEV
841TMVTARPNFQ DSIHVGFVSG LKKFTEYFTS VLCFTTPGDG PRSTPQLVRT HEDVPGPVGH
901LSFSEILDTS LKVSWQEPGE KNGILTGYRI SWEEYNRTNT RVTHYLPNVT LEYRVTGLTA
961LTTYTIEVAA MTSKGQGQVS ASTISSGVPP ELPGPPTNLG ISNIGPRSVT LQFRPGYDGK
1021TSISRWLVEA QVGVVGEGEE WLLIHQLSNE PDARSMEVPD LNPFTCYSFR MRQVNIVGTS
1081PPSQPSRKIQ TLQAPPDMAP ANVSLRTASE TSLWLRWMPL PEMEYNGNPE SVGYKIKYSR
1141SDGHGKTLSH VVQDRVERDY TIEDLEEWTE YRVQVQAFNA IGSGPWSQTV VGRTRESVPS
1201SGPTNVSALA TTSSSMLVRW SEVPEADRNG LVLGYKVMYK EKDSDTQPRF WLVEGNSSRS
1261AQLTGLGKYV LYEVQVLAFT RIGDGSPSHP PILERTLDDV PGPPMGILFP EVRTTSVRLI
1321WQPPAAPNGI ILAYQITHRL NTTTANTATV EVLAPSARQY TATGLKPESV YLFRITAQTR
1381KGWGEAAEAL VVTTEKRDRP QPPSRPMVQQ EDVRARSVLL SWEPGSDGLS PVRYYTIQTR
1441ELPSGRWALH SASVSHNASS FIVDRLKPFT SYKFRVKATN DIGDSEFSEE SESLTTLQAA
1501PDEAPTILSV TPHTTTSVLI RWQPPAEDKI NGILLGFRIR YRELLYEGLR GFTLRGINNP
1561GATWAELTSM YSMRNLSRPS LTQYELDNLN KHRRYEIRMS VYNAVGEGPS SPPQEVFVGE
1621AVPTAAPRNV VVHGATATQL DVTWEPPPLD SQNGDIQGYK IYFWEAQRGN LTERVKTLFL
1681AENSVKLKNL TGYTAYMVSV AAFNAAGDGP RSTPTQGQTQ QAAPSAPSSV KFSELTTTSV
1741NVSWEAPQFP NGILEGYRLV YEPCSPVDGV SKIVTVDVKG NSPLWLKVKD LAEGVTYRFR
1801IRAKTFTYGP EIEANVTTGP GEGAPGPPGV PIIVRYSSAI AIHWSSGDPG KGPITRYVIE
1861ARPSDEGLWD ILIKDIPKEV SSYTFSMDIL KPGVSYDFRV IAVNDYGFGT PSSPSQSVPA
1921QKANPFYEEW WFLVVIALVG LIFILLLVFV LIIRGQSKKY AKKTDSGNSA KSGALGHSEM
1981MSLDESSFPA LELNNRRLSV KNSFCRKNGL YTRSPPRPSP GSLHYSDEDV TKYNDLIPAE
2041SSSLTEKPSE ISDSQGSDSE YEVDSNHQKA HSFVNHYISD PTYYNSWRRQ QKGISRAQAY
2101SYTESDSGEP DHTTVTNSTS TQQGSLFRPK ASRTPTPQNP PNPPSQQSTL YRPPSSLAPG
2161SRAPIAGFSS FV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2172 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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