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SDCCAG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SDCCAG3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120378-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SDCCAG3 (NM_001039707), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SDCCAG3 | Antigen NY-CO-3 | NY-CO-3
Origin Species
Human
NCBI GenBank
NM_006643
SwissProt
Q96C92

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47.8 kda
Protein Type
Lysate
Amino Acid Sequence
1MSGYQRRPGA TPLSRARSLA IPDAPAFYER RSCLPQLNCE RPHGRDLDSP FFGIRPAFMC
61YVPSPVLASV GDTDFGYGKG KCSKQSPSGA HGTHFGDDRF EDLEEANPFS FREFLKTKNL
121GLSKEDPASR IYAKEASRHS LGLDHNSPPS QTGGYGLEYQ QPFFEDPTGA GDLLDEEEDE
181DTGWSGAYLP SAIEQTHPER VPAGTSPCST YLSFFSTPSE LAGPESLPSW ALSDTDSRVS
241PASPAGSPSA DFAVHGESLG DRHLRTLQIS YDALKDENSK LRRKLNEVQS FSEAQTEMVR
301TLERKLEAKM IKEESDYHDL ESVVQQVEQN LELMTKRAVK AENHVVKLKQ EISLLQAQVS
361NFQRENEALR CGQGASLTVV KQNADVALQN LRVVMNSAQA SIKQLVSGAE TLNLVAEILK
421SIDRISEVKD EEEDS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

435 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.