Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SDCCAG1 / NEMF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116464-100
Catalog Number: LS-G116464
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NEMF (NM_004713), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Antigen NY-CO-1 | Nuclear export mediator factor | NY-CO-1 | SDCCAG1 | NEMF
- Origin Species
- Human
- NCBI GenBank
- NM_004713
- SwissProt
- O60524
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 122.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKSRFSTIDL RAVLAELNAS LLGMRVNNVY DVDNKTYLIR LQKPDFKATL LLESGIRIHT61TEFEWPKNMM PSSFAMKCRK HLKSRRLVSA KQLGVDRIVD FQFGSDEAAY HLIIELYDRG121NIVLTDYEYV ILNILRFRTD EADDVKFAVR ERYPLDHARA AEPLLTLERL TEIVASAPKG181ELLKRVLNPL LPYGPALIEH CLLENGFSGN VKVDEKLETK DIEKVLVSLQ KAEDYMKTTS241NFSGKGYIIQ KREIKPCLEA DKPVEDILTY EEFHPFLFSQ HSQCPYIEFE SFDKAVDEFY301SKIEGQKIDL KALQQEKQAL KKLDNVRKDH ENRLEALQQA QEIDKLKGEL IEMNLQIVDR361AIQVVRSALA NQIDWTEIGL IVKEAQAQGD PVASAIKELK LQTNHVTMLL RNPYLLSEEE421DDDVDGDVNV EKNETEPPKG KKKKQKNKQL QKPQKNKPLL VDVDLSLSAY ANAKKYYDHK481RYAAKKTQKT VEAAEKAFKS AEKKTKQTLK EVQTVTSIQK ARKVYWFEKF LWFISSENYL541IIGGRDQQQN EIIVKRYLTP GDIYVHADLH GATSCVIKNP TGEPIPPRTL TEAGTMALCY601SAAWDARVIT SAWWVYHHQV SKTAPTGEYL TTGSFMIRGK KNFLPPSYLM MGFSFLFKVD661ESCVWRHQGE RKVRVQDEDM ETLASCTSEL ISEEMEQLDG GDTSSDEDKE EHETPVEVEL721MTQVDQEDIT LQSGRDELNE ELIQEESSED EGEYEEVRKD QDSVGEMKDE GEETLNYPDT781TIDLSHLQPQ RSIQKLASKE ESSNSSDSKS QSRRHLSAKE RREMKKKKLP SDSGDLEALE841GKDKEKESTV HIETHQNTSK NVAAVQPMKR GQKSKMKKMK EKYKDQDEED RELIMKLLGS901AGSNKEEKGK KGKKGKTKDE PVKKQPQKPR GGQRVSDNIK KETPFLEVIT HELQDFAVDD961PHDDKEEQDL DQQGNEENLF DSLTGQPHPE DVLLFAIPIC APYTTMTNYK YKVKLTPGVQ1021KKGKAAKTAL NSFMHSKEAT AREKDLFRSV KDTDLSRNIP GKVKVSAPNL LNVKRKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1076 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.