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SCYL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCYL3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111156-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCY1 like 3(SCYL3) (NM_020423), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Ezrin-binding protein PACE-1 | PACE-1 | SCY1-like 3 (S. cerevisiae) | SCYL3 | PACE1 | SCY1-like protein 3
Origin Species
Human
NCBI GenBank
NM_020423
SwissProt
Q8IZE3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
76.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSENSALKS YTLREPPFTL PSGLAVYPAV LQDGKFASVF VYKRENEDKV NKAAKHLKTL
61RHPCLLRFLS CTVEADGIHL VTERVQPLEV ALETLSSAEV CAGIYDILLA LIFLHDRGHL
121THNNVCLSSV FVSEDGHWKL GGMETVCKVS QATPEFLRSI QSIRDPASIP PEEMSPEFTT
181LPECHGHARD AFSFGTLVES LLTILNEQVS ADVLSSFQQT LHSTLLNPIP KCRPALCTLL
241SHDFFRNDFL EVVNFLKSLT LKSEEEKTEF FKFLLDRVSC LSEELIASRL VPLLLNQLVF
301AEPVAVKSFL PYLLGPKKDH AQGETPCLLS PALFQSRVIP VLLQLFEVHE EHVRMVLLSH
361IEAYVEHFTQ EQLKKVILPQ VLLGLRDTSD SIVAITLHSL AVLVSLLGPE VVVGGERTKI
421FKRTAPSFTK NTDLSLEGDP FSQPIKFPIN GLSDVKNTSE DSENFPSSSK KSEEWPDWSE
481PEEPENQTVN IQIWPREPCD DVKSQCTTLD VEESSWDDCE PSSLDTKVNP GGGITATKPV
541TSAEQKPIPA LLSLTEESMP WKSSLPQKIS LVQRGDDADQ IEPPKVSSQE RPLKVPSELG
601LGEEFTIQVK KKPVKDPEMD WFADMIPEIK PSAAFLILPE LRTEMVPKKD DVSPVMQFSS
661KFAAAEITEG EAEGWEEEGE LNWEDNNW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

688 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.