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SCUBE3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCUBE3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SCUBE3 antibodies →
$504.00each
Catalog Number: LS-G106030-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCUBE3 (NM_152753), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CEGF3 | SCUBE3
Origin Species
Human
SwissProt
Q8IX30

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSGRVPGLC LLVLLVHARA AQYSKAAQDV DECVEGTDNC HIDAICQNTP RSYKCICKSG
61YTGDGKHCKD VDECEREDNA GCVHDCVNIP GNYRCTCYDG FHLAHDGHNC LDVDECAEGN
121GGCQQSCVNM MGSYECHCRE GFFLSDNQHT CIQRPEGMNC MNKNHGCAHI CRETPKGGIA
181CECRPGFELT KNQRDCKLTC NYGNGGCQHT CDDTEQGPRC GCHIKFVLHT DGKTCIETCA
241VNNGGCDSKC HDAAIGVHCT CPVGFMLQPD RKTCKDIDEC RLNNGGCDHI CRNTVGSFEC
301SCKKGYKLLI NERNCQDIDE CSFDRTCDHI CVNTPGSFQC LCHRGYLLYG ITHCGDVDEC
361SINRGGCRFG CINTPGSYQC TCPAGQGRLH WNGKDCTEPL KCQGSPGASK AMLSCNRSGK
421KDTCALTCPS RARFLPESEN GFTVSCGTPS PRAAPARAGH NGNSTNSNHC HEAAVLSIKQ
481RASFKIKDAK CRLHLRNKGK TEEAGRITGP GGAPCSECQV TFIHLKCDSS RKGKGRRART
541PPGKEVTRLT LELEAEVRAE ETTASCGLPC LRQRMERRLK GSLKMLRKSI NQDRFLLRLA
601GLDYELAHKP GLVAGERAEP MESCRPGQHR AGTKCVSCPQ GTYYHGQTEQ CVPCPAGTFQ
661EREGQLSCDL CPGSDAHGPL GATNVTTCAG QCPPGQHSVD GFKPCQPCPR GTYQPEAGRT
721LCFPCGGGLT TKHEGAISFQ DCDTKVQCSP GHYYNTSIHR CIRCAMGSYQ PDFRQNFCSR
781CPGNTSTDFD GSTSVAQCKN RQCGGELGEF TGYIESPNYP GNYPAGVECI WNINPPPKRK
841ILIVVPEIFL PSEDECGDVL VMRKNSSPSS ITTYETCQTY ERPIAFTARS RKLWINFKTS
901EANSARGFQI PYVTYDEDYE QLVEDIVRDG RLYASENHQE ILKDKKLIKA FFEVLAHPQN
961YFKYTEKHKE MLPKSFIKLL RSKVSSFLRP YK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

992 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.