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SCUBE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCUBE2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SCUBE2 antibodies →
$725.00each
Catalog Number: LS-G130239-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCUBE2 (NM_001170690), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CEGB1 | CEGF1 | CEGP1 | SCUBE2 | Protein CEGP1
Origin Species
Human
NCBI GenBank
NM_020974

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGVAGRNRPG AAWAVLLLLL LLPPLLLLAG AVPPGRGRAA GPQEDVDECA QGLDDCHADA
61LCQNTPTSYK CSCKPGYQGE GRQCEDIDEC GNELNGGCVH DCLNIPGNYR CTCFDGFMLA
121HDGHNCLDVD ECLENNGGCQ HTCVNVMGSY ECCCKEGFFL SDNQHTCIHR SEEGLSCMNK
181DHGCSHICKE APRGSVACEC RPGFELAKNQ RDCILTCNHG NGGCQHSCDD TADGPECSCH
241PQYKMHTDGR SCLEREDTVL EVTESNTTSV VDGDKRVKRR LLMETCAVNN GGCDRTCKDT
301STGVHCSCPV GFTLQLDGKT CKDIDECQTR NGGCDHFCKN IVGSFDCGCK KGFKLLTDEK
361SCQDVDECSL DRTCDHSCIN HPGTFACACN RGYTLYGFTH CGDTNECSIN NGGCQQVCVN
421TVGSYECQCH PGYKLHWNKK DCVASCDLSC IVKRTEKRLR KAIRTLRKAV HREQFHLQLS
481GMNLDVAKKP PRTSERQAES CGVGQGHAEN QCVSCRAGTY YDGARERCIL CPNGTFQNEE
541GQMTCEPCPR PGNSGALKTP EAWNMSECGG LCQPGEYSAD GFAPCQLCAL GTFQPEAGRT
601SCFPCGGGLA TKHQGATSFQ DCETRVQCSP GHFYNTTTHR CIRCPVGTYQ PEFGKNNCVS
661CPGNTTTDFD GSTNITQCKT SSNSVTTYET CQTYERPIAF TSRSKKLWIQ FKSNEGNSAR
721GFQVPYVTYD EDYQELIEDI VRDGRLYASE NHQEILKDKK LIKALFDVLA HPQNYFKYTA
781QESREMFPRS FIRLLRSKVS RFLRPYK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

807 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.