Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SCP2 / SCPX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G130997-100
Catalog Number: LS-G130997
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Sterol carrier protein 2(SCP2) (NM_001193600), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NLTP | NSL-TP | SCP-CHI | SCP-X | Sterol carrier protein 2 | Sterol carrier protein X | SCP-2 | SCP2 | SCPX
- Origin Species
- Hu
- NCBI GenBank
- NM_002979
- SwissProt
- P22307
Protein
- Expression System
- HEK293
- Fusion Tag
- FLAG, Myc
- Molecular Weight
- 54.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSSPWEPAT LRRVFVVGVG MTKFVKPGAE NSRDYPDLAE EAGKKALADA QIPYSAVDQA61CVGYVFGVAE CVLALGFEKM SKGSLGIKFS DRTIPTDKHV DLLINKYGLS AHPVAPQMFG121YAGKEHMEKY GTKIEHFAKI GWKNHKHSVN NPYSQFQDEY SLDEVMASKE VFDFLTILQC181CPTSDGAAAA ILASEAFVQK YGLQSKAVEI LAQEMMTDLP SSFEEKSIIK MVGFDMSKEA241ARKCYEKSGL TPNDIDVIEL HDCFSTNELL TYEALGLCPE GQGATLVDRG DNTYGGKWVI301NPSGGLISKG HPLGATGLAQ CAELCWQLRG EAGKRQVPGA KVALQHNLGI GGAVVVTLYK361MGFPEAASSF RTHQIEAVPT SSASDGFKAN LVFKEIEKKL EEEGEQFVKK IGGIFAFKVK421DGPGGKEATW VVDVKNGKGS VLPNSDKKAD CTITMADSDF LALMTGKMNP QSAFFQGKLK481ITGNMGLAMK LQNLQLQPGN AKLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
503 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconj
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.