Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SCP1 / SYCP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G117520-100
Catalog Number: LS-G117520
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCP1(SYCP1) (NM_003176), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cancer/testis antigen 8 | SCP-1 | Synaptonemal complex protein 1 | SCP1 | SYCP1 | CT8 | HOM-TES-14
- Origin Species
- Human
- NCBI GenBank
- NM_003176
- SwissProt
- Q15431
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 114 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEKQKPFALF VPPRSSSSQV SAVKPQTLGG DSTFFKSFNK CTEDDFEFPF AKTNLSKNGE61NIDSDPALQK VNFLPVLEQV GNSDCHYQEG LKDSDLENSE GLSRVYSKLY KEAEKIKKWK121VSTEAELRQK ESKLQENRKI IEAQRKAIQE LQFGNEKVSL KLEEGIQENK DLIKENNATR181HLCNLLKETC ARSAEKTKKY EYEREETRQV YMDLNNNIEK MITAFEELRV QAENSRLEMH241FKLKEDYEKI QHLEQEYKKE INDKEKQVSL LLIQITEKEN KMKDLTFLLE ESRDKVNQLE301EKTKLQSENL KQSIEKQHHL TKELEDIKVS LQRSVSTQKA LEEDLQIATK TICQLTEEKE361TQMEESNKAR AAHSFVVTEF ETTVCSLEEL LRTEQQRLEK NEDQLKILTM ELQKKSSELE421EMTKLTNNKE VELEELKKVL GEKETLLYEN KQFEKIAEEL KGTEQELIGL LQAREKEVHD481LEIQLTAITT SEQYYSKEVK DLKTELENEK LKNTELTSHC NKLSLENKEL TQETSDMTLE541LKNQQEDINN NKKQEERMLK QIENLQETET QLRNELEYVR EELKQKRDEV KCKLDKSEEN601CNNLRKQVEN KNKYIEELQQ ENKALKKKGT AESKQLNVYE IKVNKLELEL ESAKQKFGEI661TDTYQKEIED KKISEENLLE EVEKAKVIAD EAVKLQKEID KRCQHKIAEM VALMEKHKHQ721YDKIIEERDS ELGLYKSKEQ EQSSLRASLE IELSNLKAEL LSVKKQLEIE REEKEKLKRE781AKENTATLKE KKDKKTQTFL LETPEIYWKL DSKAVPSQTV SRNFTSVDHG ISKDKRDYLW841TSAKNTLSTP LPKAYTVKTP TKPKLQQREN LNIPIEESKK KRKMAFEFDI NSDSSETTDL901LSMVSEEETL KTLYRNNNPP ASHLCVKTPK KAPSSLTTPG STLKFGAIRK MREDRWAVIA961KMDRKKKLKE AEKLFVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
976 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.