Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SCNN1A / ENaC Alpha Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G128316-100
Catalog Number: LS-G128316
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human epithelial Sodium Channel alpha(SCNN1A) (NM_001159575), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha hENaC | Alpha-NaCH | Alpha-ENaC | ENaCalpha | ENaCa | ENaC alpha | HENaC alpha | Alpha ENaC-2 | SCNEA | SCNN1 | BESC2 | SCNN1A
- Origin Species
- Human
- NCBI GenBank
- NM_001038
- SwissProt
- P37088
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSSIKGNKLE EQDPRPLQPI PGLMEGNKLE EQDSSPPQST PGLMKGNKRE EQGLGPEPAA61PQQPTAEEEA LIEFHRSYRE LFEFFCNNTT IHGAIRLVCS QHNRMKTAFW AVLWLCTFGM121MYWQFGLLFG EYFSYPVSLN INLNSDKLVF PAVTICTLNP YRYPEIKEEL EELDRITEQT181LFDLYKYSSF TTLVAGSRSR RDLRGTLPHP LQRLRVPPPP HGARRARSVA SSLRDNNPQV241DWKDWKIGFQ LCNQNKSDCF YQTYSSGVDA VREWYRFHYI NILSRLPETL PSLEEDTLGN301FIFACRFNQV SCNQANYSHF HHPMYGNCYT FNDKNNSNLW MSSMPGINNG LSLMLRAEQN361DFIPLLSTVT GARVMVHGQD EPAFMDDGGF NLRPGVETSI SMRKETLDRL GGDYGDCTKN421GSDVPVENLY PSKYTQQVCI HSCFQESMIK ECGCAYIFYP RPQNVEYCDY RKHSSWGYCY481YKLQVDFSSD HLGCFTKCRK PCSVTSYQLS AGYSRWPSVT SQEWVFQMLS RQNNYTVNNK541RNGVAKVNIF FKELNYKTNS ESPSVTMVTL LSNLGSQWSL WFGSSVLSVV EMAELVFDLL601VIMFLMLLRR FRSRYWSPGR GGRGAQEVAS TLASSPPSHF CPHPMSLSLS QPGPAPSPAL661TAPPPAYATL GPRPSPGGSA GASSSTCPLG GPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
692 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.