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SCML2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCML2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SCML2 antibodies →
$504.00each
Catalog Number: LS-G115532-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCML2 (NM_006089), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SCML2
Origin Species
Human
NCBI GenBank
NM_006089
SwissProt
Q9UQR0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGQTVNEDSM DVKKENQEKT PQSSTSSVQR DDFHWEEYLK ETGSISAPSE CFRQSQIPPV
61NDFKVGMKLE ARDPRNATSV CIATVIGITG ARLRLRLDGS DNRNDFWRLV DSPDIQPVGT
121CEKEGDLLQP PLGYQMNTSS WPMFLLKTLN GSEMASATLF KKEPPKPPLN NFKVGMKLEA
181IDKKNPYLIC PATIGDVKGD EVHITFDGWS GAFDYWCKYD SRDIFPAGWC RLTGDVLQPP
241GTSVPIVKNI AKTESSPSEA SQHSMQSPQK TTLILPTQQV RRSSRIKPPG PTAVPKRSSS
301VKNITPRKKG PNSGKKEKPL PVICSTSAAS LKSLTRDRGM LYKDVASGPC KIVMSTVCVY
361VNKHGNFGPH LDPKRIQQLP DHFGPGPVNV VLRRIVQACV DCALETKTVF GYLKPDNRGG
421EVITASFDGE THSIQLPPVN SASFALRFLE NFCHSLQCDN LLSSQPFSSS RGHTHSSAEH
481DKNQSAKEDV TERQSTKRSP QQTVPYVVPL SPKLPKTKEY ASEGEPLFAG GSAIPKEENL
541SEDSKSSSLN SGNYLNPACR NPMYIHTSVS QDFSRSVPGT TSSPLVGDIS PKSSPHEVKF
601QMQRKSEAPS YIAVPDPSVL KQGFSKDPST WSVDEVIQFM KHTDPQISGP LADLFRQHEI
661DGKALFLLKS DVMMKYMGLK LGPALKLCYY IEKLKEGKYS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

700 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.