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SCMH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCMH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G130038-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCMH1 (NM_001172220), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Polycomb protein SCMH1 | SCMH1 | Sex comb on midleg homolog 1 | Scml3
Origin Species
Human
NCBI GenBank
NM_012236
SwissProt
Q96GD3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKLEAQDPRN TTSTCIATVV GLTGARLRLR LDGSDNKNDF WRLVDSAEIQ PIGNCEKNGG
61MLQPPLGFRL NASSWPMFLL KTLNGAEMAP IRIFHKEPPS PSHNFFKMGM KLEAVDRKNP
121HFICPATIGE VRGSEVLVTF DGWRGAFDYW CRFDSRDIFP VGWCSLTGDN LQPPGTKVVI
181PKNPYPASDV NTEKPSIHSS TKTVLEHQPG QRGRKPGKKR GRTPKTLISH PISAPSKTAE
241PLKFPKKRGP KPGSKRKPRT LLNPPPASPT TSTPEPDTST VPQDAATIPS SAMQAPTVCI
301YLNKNGSTGP HLDKKKVQQL PDHFGPARAS VVLQQAVQAC IDCAYHQKTV FSFLKQGHGG
361EVISAVFDRE QHTLNLPAVN SITYVLRFLE KLCHNLRSDN LFGNQPFTQT HLSLTAIEYS
421HSHDRYLPGE TFVLGNSLAR SLEPHSDSMD SASNPTNLVS TSQRHRPLLS SCGLPPSTAS
481AVRRLCSRGS DRYLESRDAS RLSGRDPSSW TVEDVMQFVR EADPQLGPHA DLFRKHEIDG
541KALLLLRSDM MMKYMGLKLG PALKLSYHID RLKQGKF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

577 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.