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SCIN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCIN Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SCIN antibodies →
$504.00each
Catalog Number: LS-G102113-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCIN (SCIN) (NM_033128), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Adseverin | KIAA1905 | SCIN | Scinderin
Origin Species
Human
NCBI GenBank
NM_001112706
SwissProt
Q9Y6U3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARELYHEEF ARAGKQAGLQ VWRIEKLELV PVPQSAHGDF YVGDAYLVLH TAKTSRGFTY
61RLHFWLGKEC SQDESTAAAI FTVQMDDYLG GKPVQNRELQ GYESNDFVSY FKGGLKYKAG
121GVASGLNHVL TNDLTAKRLL HVKGRRVVRA TEVPLSWDSF NKGDCFIIDL GTEIYQWCGS
181SCNKYERLKA NQVATGIRYN ERKGRSELIV VEEGSEPSEL IKVLGEKPEL PDGGDDDDII
241ADISNRKMAK LYMVSDASGS MRVTVVAEEN PFSMAMLLSE ECFILDHGAA KQIFVWKGKD
301ANPQERKAAM KTAEEFLQQM NYSKNTQIQV LPEGGETPIF KQFFKDWRDK DQSDGFGKVY
361VTEKVAQIKQ IPFDASKLHS SPQMAAQHNM VDDGSGKVEI WRVENNGRIQ VDQNSYGEFY
421GGDCYIILYT YPRGQIIYTW QGANATRDEL TTSAFLTVQL DRSLGGQAVQ IRVSQGKEPV
481HLLSLFKDKP LIIYKNGTSK KGGQAPAPPT RLFQVRRNLA SITRIVEVDV DANSLNSNDV
541FVLKLPQNSG YIWVGKGASQ EEEKGAEYVA SVLKCKTLRI QEGEEPEEFW NSLGGKKDYQ
601TSPLLETQAE DHPPRLYGCS NKTGRFVIEE IPGEFTQDDL AEDDVMLLDA WEQIFIWIGK
661DANEVEKKES LKSAKMYLET DPSGRDKRTP IVIIKQGHEP PTFTGWFLGW DSSKW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

715 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.