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SCFD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCFD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SCFD2 antibodies →
$504.00each
Catalog Number: LS-G106180-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCFD2 (NM_152540), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SCFD2 | STXBP1L1
Origin Species
Human
SwissProt
Q8BTY8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSASGVLSFT QQGWEQVLAK VKRAVVYLDA ACAESLHWGC GSTRLLEAVG GPDCHLREFE
61PDAIGGGAKQ PKAVFVLSCL LKGRTVEILR DIICRSHFQY CVVVTTVSHA VHLTANHVPA
121AAAAEMEGQQ PVFEQLEEKL CEWMGNMNYT AEVFHVPLLL APVAPHFALT PAFASLFPLL
181PQDVHLLNSA RPDKRKLGSL GDVDSTTLTP ELLLQIRCLV SGLSSLCEHL GVREECFAVG
241SLSQVIAADL ANYAPAKNRK KTAAGRASVV FVDRTLDLTG AVGHHGDNLV EKIISALPQL
301PGHTNDVMVN MIALTALHTE EENYNVVAPG CLSQSSDTTA KALWEALLNT KHKEAVMEVR
361RHLVEAASRE NLPIKMSMGR VTPGQLMSYI QLFKNNLKAL MNHCGLLQLG LATAQTLKHP
421QTAKWDNFLA FERLLLQSIG ESAMSVVLNQ LLPMIKPVTQ RTNEDYSPEE LLILLIYIYS
481VTGELTVDKD LCEAEEKVKK ALAQVFCEES GLSPLLQKIT DWDSSINLTF HKSKIAVDEL
541FTSLRDIAGA RSLLKQFKSV YVPGNHTHQA SYKPLLKQVV EEIFHPERPD SVDIEHMSSG
601LTDLLKTGFS MFMKVSRPHP SDYPLLILFV VGGVTVSEVK MVKDLVASLK PGTQVIVLST
661RLLKPLNIPE LLFATDRLHP DLGF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

684 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.