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SCEL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCEL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SCEL antibodies →
$504.00each
Catalog Number: LS-G117053-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCEL (NM_003843), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SCEL | Sciellin
Origin Species
Human
NCBI GenBank
NM_003843
SwissProt
O95171

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSNVTLRKMS PTGNEMKSTT QGTTRKQQDF HEVNKRRTFL QDNSWIKKRP EEEKDENYGR
61VVLNRHNSHD ALDRKVNERD VPKATISRYS SDDTLDRISD RNDAAKTYKA NTLDNQLTNR
121SMSVFRSLEV TKLQPGGSLN ANTSNTIAST SATTPVKKKR QSWFPPPPPG YNASSSTGTR
181RREPGVHPPI PPKPSSPVSS TNQLRQDNRQ IHPPKPGVYT ETNRSAERNI SEELDNLIKM
241NKSLNRNQGL DSLFRANPKV EEREKRAKSL ESLIYMSTRT DKDGKGIQSL GSPIKVNQRT
301DKNEKGRQNL ESVAKVNARM NKTSRRSEDL DNATEVNPKG HENTTGKKDL DGLIKVDPET
361NKNITRGQSL DNLIKVTPEV KRSNQGSKDL NNFIKVYPGT EKSTEGGQSL DSLIKVTPER
421NRTNQGNQDL ENLIKVIPSA NKSSEQGLDE HINVSPKAVK NTDGKQDLDK LIKVNPEIFT
481NNQRNQDLAN LIKVNPAVIR NNQSQDLDNL IKVKPSALRN TNRDQNLENL IEVNSHVSEN
541KNGSSNTGAK QAGPQDTVVY TRTYVENSKS PKDGYQENIS GKYIQTVYST SDRSVIERDM
601CTYCRKPLGV ETKMILDELQ ICCHSTCFKC EICKQPLENL QAGDSIWIYR QTIHCEPCYS
661KIMAKWIP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

668 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.