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SCCPDH Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCCPDH Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112920-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCCPDH (NM_016002), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CGI-49 | RP11-439E19.2 | NET11 | SCCPDH
Origin Species
Human
NCBI GenBank
NM_016002
SwissProt
Q8NBX0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATEQRPFHL VVFGASGFTG QFVTEEVARE QVDPERSSRL PWAVAGRSRE KLQRVLEKAA
61LKLGRPTLSS EVGIIICDIA NPASLDEMAK QATVVLNCVG PYRFYGEPVI KACIENGASC
121IDISGEPQFL ELMQLKYHEK AADKGVYIIG SSGFDSIPAD LGVIYTRNKM NGTLTAVESF
181LTIHSGHEGL SIHDGTWKSA IYGFGDQSNL RKLRNVSNLK PVPLIGPKLK RRWPISYCRE
241LKGYSIPFMG SDVSVVRRTQ RYLYENLEES PVQYAAYVTV GGITSVIKLM FAGLFFLFFV
301RFGIGRQLLI KFPWFFSFGY FSKQGPTQKQ IDAASFTLTF FGQGYSRGTG TDKNKPNIKI
361CTQVKGPEAG YVATPIAMVQ AAMTLLSDAS HLPKAGGVFT PGAAFSKTKL IDRLNKHGIE
421FSVISSSEV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

429 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.