Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SCC4 / MAU2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113296-100
Catalog Number: LS-G113296
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Scc4(MAU2) (NM_015329), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MAU2 | MAU2L | KIAA0892 | Protein MAU-2 | SCC4 | Sister chromatid cohesion 4 | Mau-2
- Origin Species
- Human
- NCBI GenBank
- AB020699
- SwissProt
- Q9Y6X3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAQAAAAAQ AAAAQAAQAE AADSWYLALL GFAEHFRTSS PPKIRLCVHC LQAVFPFKPP61QRIEARTHLQ LGSVLYHHTK NSEQARSHLE KAWLISQQIP QFEDVKFEAA SLLSELYCQE121NSVDAAKPLL RKAIQISQQT PYWHCRLLFQ LAQLHTLEKD LVSACDLLGV GAEYARVVGS181EYTRALFLLS KGMLLLMERK LQEVHPLLTL CGQIVENWQG NPIQKESLRV FFLVLQVTHY241LDAGQVKSVK PCLKQLQQCI QTISTLHDDE ILPSNPADLF HWLPKEHMCV LVYLVTVMHS301MQAGYLEKAQ KYTDKALMQL EKLKMLDCSP ILSSFQVILL EHIIMCRLVT GHKATALQEI361SQVCQLCQQS PRLFSNHAAQ LHTLLGLYCV SVNCMDNAEA QFTTALRLTN HQELWAFIVT421NLASVYIREG NRHQEVLYSL LERINPDHSF PVSSHCLRAA AFYVRGLFSF FQGRYNEAKR481FLRETLKMSN AEDLNRLTAC SLVLLGHIFY VLGNHRESNN MVVPAMQLAS KIPDMSVQLW541SSALLRDLNK ACGNAMDAHE AAQMHQNFSQ QLLQDHIEAC SLPEHNLITW TDGPPPVQFQ601AQNGPNTSLA SLLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
613 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.