Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SCARA3 / APC7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112777-100
Catalog Number: LS-G112777
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCARA3 (NM_016240), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CSR1 | CSR | MSLR1 | MSRL1 | APC7 | SCARA3
- Origin Species
- Human
- NCBI GenBank
- NM_016240
- SwissProt
- Q6AZY7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKVRSAGGDG DALCVTEEDL AGDDEDMPTF PCTQKGRPGP RCSRCQKNLS LHTSVRILYL61FLALLLVAVA VLASLVFRKV DSLSEDISLT QSIYDKKLVL MQKNLQGLDP KALNNCSFCH121EAGQLGPEIR KLQEELEGIQ KLLLAQEVQL DQTLQAQEVL STTSRQISQE MGSCSFSIHQ181VNQSLGLFLA QVRGWQATTA GLDLSLKDLT QECYDVKAAV HQINFTVGQT SEWIHGIQRK241TDEETLTLQK IVTDWQNYTR LFSGLRTTST KTGEAVKNIQ ATLGASSQRI SQNSESMHDL301VLQVMGLQLQ LDNISSFLDD HEENMHDLQY HTHYAQNRTV ERFESLEGRM ASHEIEIGTI361FTNINATDNH VHSMLKYLDD VRLSCTLGFH THAEELYYLN KSVSIMLGTT DLLRERFSLL421SARLDLNVRN LSMIVEEMKA VDTQHGEILR NVTILRGAPG PPGPRGFKGD MGVKGPVGGR481GPKGDPGSLG PLGPQGPQGQ PGEAGPVGER GPVGPRGFPG LKGSKGSFGT GGPRGQPGPK541GDIGPPGPEG PPGSPGPSGP QGKPGIAGKT GSPGQRGAMG PKGEPGIQGP PGLPGPPGPP601GSQSFYTRTRPLEQKLISEEDLAANDILDYKDDDDKV
606 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.