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SCAPER Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCAPER Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G129185-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCAPER (NM_020843), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MSTP063 | SCAPER | ZNF291 | KIAA1454 | Zfp291 | Zinc finger protein 291
Origin Species
Human
NCBI GenBank
AB040887
SwissProt
Q9BY12

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
158.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMASFQRSNS HDKVRRIVAE EGRTARNLIA WSVPLESKDD DGKPKCQTGG KSKRTIQGTH
61KTTKQSTAVD CKITSSTTGD KHFDKSPTKT RHPRKIDLRA RYWAFLFDNL RRAVDEIYVT
121CESDQSVVEC KEVLMMLDNY VRDFKALIDW IQLQEKLEKT DAQSRPTSLA WEVKKMSPGR
181HVIPSPSTDR INVTSNARRS LNFGGSTGTV PAPRLAPTGV SWADKVKAHH TGSTASSEIT
241PAQSCPPMTV QKASRKNERK DAEGWETVQR GRPIRSRSTA VMPKVSLATE ATRSKDDSDK
301ENVCLLPDES IQKGQFVGDG TSNTIESHPK DSLHSCDHPL AEKTQFTVST LDDVKNSGSI
361RDNYVRTSEI SAVHIDTECV SVMLQAGTPP LQVNEEKFPA EKARIENEMD PSDISNSMAE
421VLAKKEELAD RLEKANEEAI ASAIAEEEQL TREIEAEENN DINIETDNDS DFSASMGSGS
481VSFCGMSMDW NDVLADYEAR ESWRQNTSWG DIVEEEPARP PGHGIHMHEK LSSPSRKRTI
541AESKKKHEEK QMKAQQLREK LREEKTLKLQ KLLEREKDVR KWKEELLDQR RRMMEEKLLH
601AEFKREVQLQ AIVKKAQEEE AKVNEIAFIN TLEAQNKRHD VLSKLKEYEQ RLNELQEERQ
661RRQEEKQARD EAVQERKRAL EAERQARVEE LLMKRKEQEA RIEQQRQEKE KAREDAARER
721ARDREERLAA LTAAQQEAME ELQKKIQLKH DESIRRHMEQ IEQRKEKAAE LSSGRHANTD
781YAPKLTPYER KKQCSLCNVL ISSEVYLFSH VKGRKHQQAV RENTSIQGRE LSDEEVEHLS
841LKKYIIDIVV ESTAPAEALK DGEERQKNKK KAKKIKARMN FRAKEYESLM ETKNSGSDSP
901YKAKLQRLAK DLLKQVQVQD SGSWANNKVS ALDRTLGEIT RILEKENVAD QIAFQAAGGL
961TALEHILQAV VPATNVNTVL RIPPKSLCNA INVYNLTCNN CSENCSDVLF SNKITFLMDL
1021LIHQLTVYVP DENNTILGRN TNKQVFEGLT TGLLKVSAVV LGCLIANRPD GNCQPATPKI
1081PTQEMKNKPS QGDPFNNRVQ DLISYVVNMG LIDKLCACFL SVQGPVDENP KMAIFLQHAA
1141GLLHAMCTLC FAVTGRSYSI FDNNRQDPTG LTAALQATDL AGVLHMLYCV LFHGTILDPS
1201TASPKENYTQ NTIQVAIQSL RFFNSFAALH LPAFQSIVGA EGLSLAFRHM ASSLLGHCSQ
1261VSCESLLHEV IVCVGYFTVN HPDNQVIVQS GRHPTVLQKL CQLPFQYFSD PRLIKVLFPS
1321LIAACYNNHQ NKIILEQEMS CVLLATFIQD LAQTPGQAEN QPYQPKGKCL GSQDYLELAN
1381RFPQQAWEEA RQFFLKKEKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1400 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.