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SCAI Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCAI Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105294-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SCAI (NM_173690), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Protein SCAI | SCAI | NET40 | C9orf126
Origin Species
Human
SwissProt
Q8N9R8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVRGARQPQQ PRSRLAPRLT GTVEKPPRKR RSSIRGSGDS SHSQSQGERY QHFTEKTEFA
61LKEIMSSGGA EDDIPQGERK TVTDFCYLLD KSKQLFNGLR DLPQYGQKQW QSYFGRTFDV
121YTKLWKFQQQ HRQVLDNRYG LKRWQIGEIA SKIGQLYYHY YLRTSETSYL NEAFSFYSAI
181RQRSYYSQVN KEDRPELVVK KLRYYARFIV VCLLLNKMDV VKDLVKELSD EIEDYTHRFN
241TEDQVEWNLV LQEVAAFIEA DPVMVLNDDN TIVITSNRLA ETGAPLLEQG MIVGQLSLAD
301ALIIGNCNNQ VKFSELTVDM FRMLQALERE PMNLASQMNK PGMQESADKP TRRENPHKYL
361LYKPTFSQLY TFLAASFKEL PANSVLLIYL SATGVFPTGR SDSEGPYDFG GVLTNSNRDI
421INGDAIHKRN QSHKEMHCLH PGDLYPFTRK PLFIIVDSSN SVAYKNFTNL FGQPLVCLLS
481PTAYPKALQD QSQRGSLFTL FLNNPLMAFL FVSGLSSMRR GLWEKCQEYL RKINRDIAQL
541LTHSRSIDQA FLQFFGDEFL RLLLTRFIFC SATMRMHKIF RETRNYPESY PQLPRDETVE
601NPHLQKHILE LASILDVRNV FFENTIDDY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

629 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.