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SCAF4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SCAF4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SCAF4 antibodies →
$725.00each
Catalog Number: LS-G126718-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SFRS15(SCAF4) (NM_001145444), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1172 | SFRS15 | SRA4 | SCAF4
Origin Species
Human
NCBI GenBank
AA883099

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDAVNAFNQE LFSLMDMKPP ISRAKMILIT KAAIKAIKCK PEYKVPGLYV IDSIVRQSRH
61QFGTDKDVFG PRFSKNITAT FQYLYLCPSE DKSKIVRVLN LWQKNGVFKI EIIQPLLDMA
121AGTSNAAPVA ENVTNNEGSP PPPVKVSSEP PTQATPNSVP AVPQLPSSDA FAAVAQLFQT
181TQGQQLQQIL QTFQQPPKPQ SPALDNAVMA QVQAITAQLK TTPTQPSEQK AAFPPPEQKT
241AFDKKLLDRF DYDDEPEAVE ESKKEDTTAV TTTAPAAAVP PAPTATVPAA AAPAAASPPP
301PQAPFGFPGD GMQQPAYTQH QNMDQFQPRM MGIQQDPMHH QVPLPPNGQM PGFGLLPTPP
361FPPMAQPVIP PTPPVQQPFQ ASFQAQNEPL TQKPHQQEME VEQPCIQEVK RHMSDNRKSR
421SRSASRSPKR RRSRSGSRSR RSRHRRSRSR SRDRRRHSPR SRSQERRDRE KERERRQKGL
481PQVKPETASV CSTTLWVGQL DKRTTQQDVA SLLEEFGPIE SINMIPPRGC AYIVMVHRQD
541AYRALQKLSR GNYKVNQKSI KIAWALNKGI KADYKQYWDV ELGVTYIPWD KVKPEELESF
601CEGGMLDSDT LNPDWKGIPK KPENEVAQNG GAETSHTEPV SPIPKPLPVP VPPIPVPAPI
661TVPPPQVPPH QPGPPVVGAL QPPAFTPPLG IPPPGFGPGV PPPPPPPPFL RPGFNPMHLP
721PGFLPPGPPP PITPPVSIPP PHTPPISIPN STIAGINEDT TKDLSIGNPI PTVVSGARGN
781AESGDSVKMY GSAVPPAAPT NLPTPPVTQP VSLLGTQGVA PGPVIGLQAP STGLLGARPG
841LIPLQRPPGM PPPHLQRFPL MPPRPMPPHM MHRGPPPGPG GFAMPPPHGM KGPFPPHGPF
901VRPGGMPGLG GPGPGPGGPE DRDGRQQPPQ QPQQQPQPQA PQQPQQQQQQ QPPPSQQPPP
961TQQQPQQFRN DNRQQFNSGR DQERFGRRSF GNRVENDRER YGNRNDDRDN SNRDRREWGR
1021RSPDRDRHRD LEERNRRSSG HRDRERDSRD RESRREKEEA RGKEKPEVTD RAGGNKTVEP
1081PISQVGNVDT ASELEKGVSE AAVLKPSEEL PAEATSSVEP EKDSGSAAEA PR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1132 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.