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SBSN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SBSN Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G128255-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SBSN (NM_001166034), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HLAR698 | UNQ698 | SBSN | Suprabasin
Origin Species
Human
SwissProt
Q6UWP8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHLARLVGSC SLLLLLGALS GWAASDDPIE KVIEGINRGL SNAEREVGKA LDGINSGITH
61AGREVEKVFN GLSNMGSHTG KELDKGVQGL NHGMDKVAHE INHGIGQAGK EAEKLGHGVN
121NAAGQVGKEA DKLIHHGVHH GANQAGSEAG KFGQGVDNAA GQAGNEAGRF GQGVHHAAGQ
181AGNEAGRFGQ GVHHAAGQAG NEAGRFGQGA HHGLSEGWKE TEKFGQGIHH AAGQVGKEAE
241KFGQGAHHAA GQAGNEAGRF GQGVHHGLSE GWKETEKFGQ GVHHTAGQVG KEAEKFGQGA
301HHAAGQAGNE AGRFGQGAHH AAGQAGNEAG RFGQGVHHGL SEGWKETEKF GQGVHHAASQ
361FGKETEKLGH GVHHGVNEAW KEAEKFGQGV HHAASQVGKE EDRVVQGLHH GVSQAGREAG
421QFGHDIHHTA GQAGKEGDIA VHGVQPGVHE AGKEAGQFGQ GVHHTLEQAG KEADKAVQGF
481HTGVHQAGKE AEKLGQGVNH AADQAGKEVE KLGQGAHHAA GQAGKELQNA HNGVNQASKE
541ANQLLNGNHQ SGSSSHQGGA TTTPLASGAS VNTPFINLPA LWRSVANIMP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

590 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.