Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SAXO2 / FAM154B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121907-100
Catalog Number: LS-G121907
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM154B(SAXO2) (NM_001008226), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGAKSMRSWC LCQICSCGSD YCPYEIVKQP RHVPEEYKPK QGKIDLGTTY KRDLNSYKVQ61PVAIVRPLER QVKKGKLDTV PTYKDDYRAW DLHKSELYKP EQTYHPPTVK FGNSTTFQDD121FVPQEIKPRQ SFKPSSVVKR STAPFNGITS HRLDYIPHQL ELKFERPKEV YKPTDQRFED181LTTHRCDFQG LIGETAKLCR PVHTRVTQNA LFEGSTEFRE SFQPWEIPPP EVKKVPEYVP241PTGSMLLNST SHLDYVPYQA NHVVPIRPVS QKRSNNFPFQ GKSIMKEDFP AWESCRQGLI301KKQQQIPNPS GKFDGLSTFR SHYVPHELIP TESCKPLNIA FKSSVPFDDV TMYSVEYTPK361RQEICPASYP SPPGYIFDNT NSQGHKFFRK IIPAVKAFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
398 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.