Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SAXO1 / FAM154A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105703-100
Catalog Number: LS-G105703
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human FAM154A(SAXO1) (NM_153707), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FAM154A | Protein FAM154A | C9orf138
- Origin Species
- Human
- NCBI GenBank
- AA442292
- SwissProt
- Q8IYX7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKTKCICELC SCGRHHCPHL PTKIYDETEK PCLLSEYTEN YPFYHSYLPR ESFKPRREYQ61KGSIPMEGLT TSRRDFGPHK VAPVKVHQYD QFVPSEENMD LLTTYKKDYN PYPVCRVDPI121KPRDSKYPCS DKMECLPTYK ADYLPWNQPR REPLRLEHKY QPASVRFDNR TTHQDDYPIK181GLVKTISCKP LAMPKLCNIP LEDVTNYKMS YVAHPVEKRF VHEAEKFRPC EIPFESLTTQ241KQSYRGLMGE PAKSLKPLAR PPGLDMPFCN TTEFRDKYQA WPMPRMFSKA PITYVPPEDR301MDLLTTVQAH YTCPKGAPAQ SCRPALQIKK CGRFEGSSTT KDDYKQWSSM RTEPVKPVPQ361LDLPTEPLDC LTTTRAHYVP HLPINTKSCK PHWSGPRGNV PVESQTTYTI SFTPKEMGRC421LASYPEPPGY TFEEVDALGH RIYKPVSQAG SQQSSHLSVD DSENPNQREL EVLATRTRPLEQKLISEEDLAANDILDYKDDDDKV
474 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.