LSBio
SART3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SART3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SART3 antibodies →
$504.00each
Catalog Number: LS-G113749-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SART3 (NM_014706), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DSAP1 | KIAA0156 | p100 | p110(nrb) | RP11-13G14 | SART-3 | SART3 | TIP110 | HSART-3 | p110
Origin Species
Human
NCBI GenBank
NM_014706
SwissProt
Q15020

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
109.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATAAETSAS EPEAESKAGP KADGEEDEVK AARTRRKVLS RAVAAATYKT MGPAWDQQEE
61GVSESDGDEY AMASSAESSP GEYEWEYDEE EEKNQLEIER LEEQLSINVY DYNCHVDLIR
121LLRLEGELTK VRMARQKMSE IFPLTEELWL EWLHDEISMA QDGLDREHVY DLFEKAVKDY
181ICPNIWLEYG QYSVGGIGQK GGLEKVRSVF ERALSSVGLH MTKGLALWEA YREFESAIVE
241AARLEKVHSL FRRQLAIPLY DMEATFAEYE EWSEDPIPES VIQNYNKALQ QLEKYKPYEE
301ALLQAEAPRL AEYQAYIDFE MKIGDPARIQ LIFERALVEN CLVPDLWIRY SQYLDRQLKV
361KDLVLSVHNR AIRNCPWTVA LWSRYLLAME RHGVDHQVIS VTFEKALNAG FIQATDYVEI
421WQAYLDYLRR RVDFKQDSSK ELEELRAAFT RALEYLKQEV EERFNESGDP SCVIMQNWAR
481IEARLCNNMQ KARELWDSIM TRGNAKYANM WLEYYNLERA HGDTQHCRKA LHRAVQCTSD
541YPEHVCEVLL TMERTEGSLE DWDIAVQKTE TRLARVNEQR MKAAEKEAAL VQQEEEKAEQ
601RKRARAEKKA LKKKKKIRGP EKRGADEDDE KEWGDDEEEQ PSKRRRVENS IPAAGETQNV
661EVAAGPAGKC AAVDVEPPSK QKEKAASLKR DMPKVLHDSS KDSITVFVSN LPYSMQEPDT
721KLRPLFEACG EVVQIRPIFS NRGDFRGYCY VEFKEEKSAL QALEMDRKSV EGRPMFVSPC
781VDKSKNPDFK VFRYSTSLEK HKLFISGLPF SCTKEELEEI CKAHGTVKDL RLVTNRAGKP
841KGLAYVEYEN ESQASQAVMK MDGMTIKENI IKVAISNPPQ RKVPEKPETR KAPGGPMLLP
901QTYGARGKGR TQLSLLPRAL QRPSAAAPQA ENGPAAAPAV AAPAATEAPK MSNADFAKLF
961LRK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

963 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.