Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SAPCD2 / C9orf140 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128950-100
Catalog Number: LS-G128950
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C9orf140(SAPCD2) (NM_178448), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_178448
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 42.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MAERGRVPPP APAPSTEGLP RAFLQSLRTL FDILDDRRRG CVHLREIESR WQGTDARELP61RGVLEGLRQV APASGYLTFE RFVAGLRTSL LSADGGPRDP TRAPARPGDQ PPPPPQRLVF121APADEPRTVL ERKPLPLGVR APLAGPSAAA RSPEQLCAPA EAAPCPAEPE RSQSAALEPS181SSADAGAVAC RALEADSGDA RRAPRARGER RRHTIASGVD CGLLKQMKEL EQEKEVLLQG241LEMMARGRDW YQQQLQRVQE RQRRLGQSRA SADFGAAGSP RPLGRLLPKV QEVARCLGEL301LAAACASRAL PPSSSGPPCP ALTSTSPPVW QQQTILMLKE QNRLLTQEVT EKSERITQLE361QEKSALIKQL FEARALSQQD GGPLDSTFITRTRPLEQKLISEEDLAANDILDYKDDDDKV
389 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.