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SAP130 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SAP130 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SAP130 antibodies →
$725.00each
Catalog Number: LS-G109933-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SAP130 (NM_024545), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SAP130 | MSin3A-associated protein 130
Origin Species
Human
NCBI GenBank
NM_024545
SwissProt
Q9H0E3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
110.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGPPRHPQAG EIEAGGAGGG RRLQVEMSSQ QFPRLGAPST GLSQAPSQIA NSGSAGLINP
61AATVNDESGR DSEVSAREHM SSSSSLQSRE EKQEPVVVRP YPQVQMLSTH HAVASATPVA
121VTAPPAHLTP AVPLSFSEGL MKPPPKPTMP SRPIAPAPPS TLSLPPKVPG QVTVTMESSI
181PQASAIPVAT ISGQQGHPSN LHHIMTTNVQ MSIIRSNAPG PPLHIGASHL PRGAAAAAVM
241SSSKVTTVLR PTSQLPNAAT AQPAVQHIIH QPIQSRPPVT TSNAIPPAVV ATVSATRAQS
301PVITTTAAHA TDSALSRPTL SIQHPPSAAI SIQRPAQSRD VTTRITLPSH PALGTPKQQL
361HTMAQKTIFS TGTPVAAATV APILATNTIP SATTAGSVSH TQAPTSTIVT MTVPSHSSHA
421TAVTTSNIPV AKVVPQQITH TSPRIQPDYP AERSSLIPIS GHRASPNPVA METRSDNRPS
481VPVQFQYFLP TYPPSAYPLA AHTYTPITSS VSTIRQYPVS AQAPNSAITA QTGVGVASTV
541HLNPMQLMTV DASHARHIQG IQPAPISTQG IQPAPIGTPG IQPAPLGTQG IHSATPINTQ
601GLQPAPMGTQ QPQPEGKTSA VVLADGATIV ANPISNPFSA APAATTVVQT HSQSASTNAP
661AQGSSPRPSI LRKKPATDGA KPKSEIHVSM ATPVTVSMET VSNQNNDQPT IAVPPTAQQP
721PPTIPTMIAA ASPPSQPAVA LSTIPGAVPI TPPITTIAAA PPPSVTVGGS LSSVLGPPVP
781EIKVKEEVEP MDIMRPVSAV PPLATNTVSP SLALLANNLS MPTSDLPPGA SPRKKPRKQQ
841HVISTEEGDM METNSTDDEK STAKSLLVKA EKRKSPPKEY IDEEGVRYVP VRPRPPITLL
901RHYRNPWKAA YHHFQRYSDV RVKEEKKAML QEIANQKGVS CRAQGWKVHL CAAQLLQLTN
961LEHDVYERLT NLQEGIIPKK KAATDDDLHR INELIQGNMQ RCKLVMDQIS EARDSMLKVL
1021DHKDRVLKLL NKNGTVKKVS KLKRKEKV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1048 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.