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SAMD4B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SAMD4B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112042-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SAMD4B (NM_018028), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HSmaug2 | Protein Smaug homolog 2 | SAMD4B | Smaug homolog B | SMAUG2 | Smaug 2 | SMGB
Origin Species
Human
NCBI GenBank
NM_018028
SwissProt
Q5PRF9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMFRDQVGIL AGWFKGWNEC EQTVALLSLL KRVTRTQARF LQLCLEHSLA DCNDIHLLES
61EANSAAIVSQ WQQESKEKVV SLLLSHLPLL QPGNTEAKSE YMRLLQKVLA YSIESNAFIE
121ESRQLLSYAL IHPATTLEDR NALALWLSHL EERLASGFRS RPEPSYHSRQ GSDEWGGPAE
181LGPGEAGPGW QDKPPRENGH VPFHPSSSVP PAINSIGSNA NTGLPCQIHP SPLKRSMSLI
241PTSPQVPGEW PSPEELGARA AFTTPDHAPL SPQSSVASSG SEQTEEQGSS RNTFQEDGSG
301MKDVPSWLKS LRLHKYAALF SQMSYEEMMT LTEQHLESQN VTKGARHKIA LSIQKLRERQ
361SVLKSLEKDV LEGGNLRNAL QELQQIIITP IKAYSVLQAT VAAATTTPTA KDGAPGEPPL
421PGAEPPLAHP GTDKGTEAKD PPAVENYPPP PAPAPTDGSE PAPAPVADGD IPSQFTRVMG
481KVCTQLLVSR PDEENITSYL QLIEKCLTHE AFTETQKKRL LSWKQQVLKL LRTFPRKAAL
541EMQNYRQQKG WAFGSNSLPI AGSVGMGVAR RTQRQFPMPP RALPPGRMGL LSPSGIGGVS
601PRHALTSPSL GGQGRQNLWF ANPGGSNSMP SQSRSSVQRT HSLPVHSSPQ AILMFPPDCP
661VPGPDLEINP TLESLCLSMT EHALGDGTDK TSTI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

694 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.