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SAMD4A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SAMD4A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113111-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SAMD4A (NM_015589), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DKFZP434H0350 | KIAA1053 | SAMD4 | SAMD4A | SMAUG1 | SMG | Smaug 1 | SMAUG | SMGA | HSmaug1 | Protein Smaug homolog 1
Origin Species
Human
NCBI GenBank
AB028976
SwissProt
Q9UPU9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFRDQVGVLA GWFKGWNECE QTVALLSLLK RVSQTQARFL QLCLEHSLAD CAELHVLERE
61ANSPGIINQW QQESKDKVIS LLLTHLPLLK PGNLDAKVEY MKLLPKILAH SIEHNQHIEE
121SRQLLSYALI HPATSLEDRS ALAMWLNHLE DRTSTSFGGQ NRGRSDSVDY GQTHYYHQRQ
181NSDDKLNGWQ NSRDSGICIN ASNWQDKSMG CENGHVPLYS SSSVPTTINT IGTSTSTILS
241GQAHHSPLKR SVSLTPPMNV PNQPLGHGWM SHEDLRARGP QCLPSDHAPL SPQSSVASSG
301SGGSEHLEDQ TTARNTFQEE GSGMKDVPAW LKSLRLHKYA ALFSQMTYEE MMALTECQLE
361AQNVTKGARH KIVISIQKLK ERQNLLKSLE RDIIEGGSLR IPLQELHQMI LTPIKAYSSP
421STTPEARRRE PQAPRQPSLM GPESQSPDCK DGAAATGATA TPSAGASGGL QPHQLSSCDG
481ELAVAPLPEG DLPGQFTRVM GKVCTQLLVS RPDEENISSY LQLIDKCLIH EAFTETQKKR
541LLSWKQQVQK LFRSFPRKTL LDISGYRQQR NRGFGQSNSL PTAGSVGGGM GRRNPRQYQI
601PSRNVPSARL GLLGTSGFVS SNQRNTTATP TIMKQGRQNL WFANPGGSNS MPSRTHSSVQ
661RTRSLPVHTS PQNMLMFQQP EFQLPVTEPD INNRLESLCL SMTEHALGDG VDRTSTI
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

717 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.