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RYK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RYK Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RYK antibodies →
$725.00each
Catalog Number: LS-G122162-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RYK (NM_001005861), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
D3S3195 | Hydroxyaryl-protein kinase | JTK5 | JTK5A protein tyrosine kinase | RYK1 | RYK | Vik | Tyrosine-protein kinase RYK | Receptor-like tyrosine kinase | JTK5A | Tyrosine kinase ryk
Origin Species
Human
NCBI GenBank
NM_002958
SwissProt
P34925

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRGAARLGRP GRSCLPGARG LRAPPPPPLL LLLALLPLLP APGAAAAPAP RPPELQSASA
61GPSVSLYLSE DEVRRLIGLD AELYYVRNDL ISHYALSFSL LVPSETNFLH FTWHAKSKVE
121YKLGFQVDNV LAMDMPQVNI SVQGEVPRTL SVFRVELSCT GKVDSEVMIL MQLNLTVNSS
181KNFTVLNFKR RKMCYKKLEE VKTSALDKNT SRTIYDPVHA APTTSTRVFY ISVGVCCAVI
241FLVAIILAVL HLHSMKRIEL DDSISASSSS QGLSQPSTQT TQYLRADTPN NATPITSSLG
301YPTLRIEKND LRSVTLLEAK GKVKDIAISR ERITLKDVLQ EGTFGRIFHG ILIDEKDPNK
361EKQAFVKTVK DQASEIQVTM MLTESCKLRG LHHRNLLPIT HVCIEEGEKP MVILPYMNWG
421NLKLFLRQCK LVEANNPQAI SQQDLVHMAI QIACGMSYLA RREVIHKDLA ARNCVIDDTL
481QVKITDNALS RDLFPMDYHC LGDNENRPVR WMALESLVNN EFSSASDVWA FGVTLWELMT
541LGQTPYVDID PFEMAAYLKD GYRIAQPINC PDELFAVMAC CWALDPEERP KFQQLVQCLT
601EFHAALGAYV
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

610 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.