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RXFP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RXFP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RXFP3 antibodies →
$725.00each
Catalog Number: LS-G101450-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Relaxin 3 receptor 1(RXFP3) (NM_016568), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GPCR135 | Relaxin-3 receptor 1 | Relaxin 3 receptor 1 | RXFPR3 | RLN3R1 | SALPR | RLN3 receptor 1 | RXFP3
Origin Species
Human
NCBI GenBank
NM_016568
SwissProt
Q9NSD7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQMADAATIA TMNKAAGGDK LAELFSLVPD LLEAANTSGN ASLQLPDLWW ELGLELPDGA
61PPGHPPGSGG AESADTEARV RILISVVYWV VCALGLAGNL LVLYLMKSMQ GWRKSSINLF
121VTNLALTDFQ FVLTLPFWAV ENALDFKWPF GKAMCKIVSM VTSMNMYASV FFLTAMSVTR
181YHSVASALKS HRTRGHGRGD CCGRSLGDSC CFSAKALCVW IWALAALASL PSAIFSTTVK
241VMGEELCLVR FPDKLLGRDR QFWLGLYHSQ KVLLGFVLPL GIIILCYLLL VRFIADRRAA
301GTKGGAAVAG GRPTGASARR LSKVTKSVTI VVLSFFLCWL PNQALTTWSI LIKFNAVPFS
361QEYFLCQVYA FPVSVCLAHS NSCLNPVLYC LVRREFRKAL KSLLWRIASP SITSMRPFTA
421TTKPEHEDQG LQAPAPPHAA AEPDLLYYPP GVVVYSGGRY DLLPSSSAY
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

469 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.