Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RWDD2B / C21orf6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112464-100
Catalog Number: LS-G112464
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RWDD2B (NM_016940), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C21orf6 | GL011 | RWDD2B | RWD domain containing 2B
- Origin Species
- Human
- NCBI GenBank
- NM_016940
- SwissProt
- P57060
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKIELSMQPW NPGYSSEGAT AQETYTCPKM IEMEQAEAQL AELDLLASMF PGENELIVND61QLAVAELKDC IEKKTMEGRS SKVYFTINMN LDVSDEKMAM FSLACILPFK YPAVLPEITV121RSVLLSRSQQ TQLNTDLTAF LQKHCHGDVC ILNATEWVRE HASGYVSRDT SSSPTTGSTV181QSVDLIFTRL WIYSHHIYNK CKRKNILEWA KELSLSGFSM PGKPGVVCVE GPQSACEEFW241SRLRKLNWKR ILIRHREDIP FDGTNDETER QRKFSIFEEK VFSVNGARGN HMDFGQLYQF301LNTKGCGDVF QMFFGVEGQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
319 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.