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RUNDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RUNDC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RUNDC1 antibodies →
$504.00each
Catalog Number: LS-G105406-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RUNDC1 (NM_173079), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RUN domain containing 1 | RUNDC1
Origin Species
Human
NCBI GenBank
NM_173079
SwissProt
Q96C34

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAIEAAAEP VTVVAAVGPK AKDEEEEEEE PLPPCEAVRW APVGAVAEAR PGATAFLEEA
61TAEEPGAAPG SPPDSSGRTL RRLRAERRRL DSALLALSSH FAQVQFRLRQ VVRGAPAEQQ
121RLLRELEDFA FRGCPHVLGY EGPGDPASDE GDGLPGDRPR LRGEDQSEQE KQERLETQRE
181KQKELILQLK TQLDDLETFA YQEGSYDSLP QSVVLERQRV IIDELIKKLD MNLNEDISSL
241STEELRQRVD AAVAQIVNPA RVKEQLVEQL KTQIRDLEMF INFIQDEVGS PLQTGGGHCE
301CKAGGKTGNG CSRTGSSRTP PGNSKTKAED VKKVRETGLH LMRRALAVLQ IFAVSQFGCA
361TGQIPPTLWQ RVQADRDYSP LLKRLEVSVD RVKQLAMRQQ PHDHVITSAN LQDLSLGGKD
421ELTMAVRKEL TVAVRDLLAH GLYASSPGMS LVMAPIACLL PAFSSAPEAM HPWELFVKYY
481HAKNGRAYVE SPARKLSQSF ALPVTGGTVV TPKQSLLTAI HMVLTEHDPF KRSADSELKA
541LVCMALNEQR LVSWVNLICK SGSLIEPHYQ PWSYMAHTGF ESALNLLSRL SSLKFSLPVD
601LAVRQLKNIK DAF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

613 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.