Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RUFY3 / RIPX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G125103-100
Catalog Number: LS-G125103
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RIPX(RUFY3) (NM_001130709), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA0871 | Protein RUFY3 | Rap2 interacting protein x | Rap2-interacting protein x | RIPX | Single axon-regulated protein | SINGAR1 | RUFY3 | Singar | Single axon-related 1
- Origin Species
- Human
- NCBI GenBank
- NM_014961
- SwissProt
- Q7L099
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAETPPPPTA GAESCSEEPA RGGEWRPEEP RRAPAGGTDR EGEAGPPPAS PAGQSEPDSP61VAAPFFLLYP GDGGAGFGVR PPPQQQRSWR TPPSPGSPLP FLLLSYPSGG GGSSGSGKHH121PNYLMANERM NLMNMAKLSI KGLIESALNL GRTLDSDYAP LQQFFVVMEH CLKHGLKAKK181TFLGQNKSFW GPLELVEKLV PEAAEITASV KDLPGLKTPV GRGRAWLRLA LMQKKLSEYM241KALINKKELL SEFYEPNALM MEEEGAIIAG LLVGLNVIDA NFCMKGEDLD SQVGVIDFSM301YLKDGNSSKG TEGDGQITAI LDQKNYVEEL NRHLNATVNN LQAKVDALEK SNTKLTEELA361VANNRIITLQ EEMERVKEES SYILESNRKG PKQDRTAEGQ ALSEARKHLK EETQLRLDVE421KELEMQISMR QEMELAMKML EKDVCEKQDA LVSLRQQLDD LRALKHELAF KLQSSDLGVK481QKSELNSRLE EKTNQMAATI KQLEQRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
506 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.