LSBio
RUFY2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RUFY2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RUFY2 antibodies →
$725.00each
Catalog Number: LS-G112070-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RUFY2 (NM_017987), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Antigen MU-RMS-40.17 | KIAA1537 | RABIP4R | ZFYVE13 | RUFY2
Origin Species
Human
NCBI GenBank
NM_017987
SwissProt
Q8WXA3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTFQVWGWRR EDASQVLAWV PDAEGGRRGM LTRRSLATKD PTAVERANLL NMAKLSIKGL
61IESALSFGRT LDSDYPPLQQ FFVVMEHCLK HGLKVRKSFL SYNKTIWGPL ELVEKLYPEA
121EEIGASVRDL PGLKTPLGRA RAWLRLALMQ KKMADYLRCL IIQRDLLSEF YEYHALMMEE
181EGAVIVGLLV GLNVIDANLC VKGEDLDSQV GVIDFSMYLK NEEDIGNKER NVQIAAILDQ
241KNYVEELNRQ LNSTVSSLHS RVDSLEKSNT KLIEELAIAK NNIIKLQEEN HQLRSENKLI
301LMKTQQHLEV TKVDVETELQ TYKHSRQGLD EMYNEARRQL RDESQLRQDV ENELAVQVSM
361KHEIELAMKL LEKDIHEKQD TLIGLRQQLE EVKAINIEMY QKLQGSEDGL KEKNEIIARL
421EEKTNKITAA MRQLEQRLQQ AEKAQMEAED EDEKYLQECL SKSDSLQKQI SQKEKQLVQL
481ETDLKIEKEW RQTLQEDLQK EKDALSHLRN ETQQIISLKK EFLNLQDENQ QLKKIYHEQE
541QALQELGNKL SESKLKIEDI KEANKALQGL VWLKDKEATH CKLCEKEFSL SKRKHHCRNC
601GEIFCNACSD NELPLPSSPK PVRVCDSCHA LLIQRCSSNL P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

641 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.