Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RTN4RL1 / NGRH2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104619-100
Catalog Number: LS-G104619
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RTN4RL1 (NM_178568), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NgR3 | NGRL2 | Nogo receptor-like 2 | Reticulon-4 receptor-like 1 | RTN4RL1 | NGRH2 | Nogo-66 receptor homolog 2 | Reticulon 4 receptor-like 1
- Origin Species
- Human
- SwissProt
- Q86UN2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLRKGCCVEL LLLLVAAELP LGGGCPRDCV CYPAPMTVSC QAHNFAAIPE GIPVDSERVF61LQNNRIGLLQ PGHFSPAMVT LWIYSNNITY IHPSTFEGFV HLEELDLGDN RQLRTLAPET121FQGLVKLHAL YLYKCGLSAL PAGVFGGLHS LQYLYLQDNH IEYLQDDIFV DLVNLSHLFL181HGNKLWSLGP GTFRGLVNLD RLLLHENQLQ WVHHKAFHDL RRLTTLFLFN NSLSELQGEC241LAPLGALEFL RLNGNPWDCG CRARSLWEWL QRFRGSSSAV PCVSPGLRHG QDLKLLRAED301FRNCTGPASP HQIKSHTLTT TDRAARKEHH SPHGPTRSKG HPHGPRPGHR KPGKNCTNPR361NRNQISKAGA GKQAPELPDY APDYQHKFSF DIMPTARPKR KGKCARRTPI RAPSGVQQAS421SASSLGASLL AWTLGLAVTL RTRTRPLEQKLISEEDLAANDILDYKDDDDKV
441 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.