Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RTN2 / Reticulon 2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115844-100
Catalog Number: LS-G115844
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Reticulon 2(RTN2) (NM_005619), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NSPLI | NSP2 | Reticulon 2 | RTN2 | SPG12 | NSP-like protein 1 | NSP-like protein I | NSPL1 | Reticulon-2
- Origin Species
- Human
- NCBI GenBank
- NM_005619
- SwissProt
- O75298
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGQVLPVFAH CKEAPSTASS TPDSTEGGND DSDFRELHTA REFSEEDEEE TTSQDWGTPR61ELTFSYIAFD GVVGSGGRRD STARRPRPQG RSVSEPRDQH PQPSLGDSLE SIPSLSQSPE121PGRRGDPDTA PPSERPLEDL RLRLDHLGWV ARGTGSGEDS STSSSTPLED EEPQEPNRLE181TGEAGEELDL RLRLAQPSSP EVLTPQLSPG SGTPQAGTPS PSRSRDSNSG PEEPLLEEEE241KQWGPLEREP VRGQCLDSTD QLEFTVEPRL LGTAMEWLKT SLLLAVYKTV PILELSPPLW301TAIGWVQRGP TPPTPVLRVL LKWAKSPRSS GVPSLSLGAD MGSKVADLLY WKDTRTSGVV361FTGLMVSLLC LLHFSIVSVA AHLALLLLCG TISLRVYRKV LQAVHRGDGA NPFQAYLDVD421LTLTREQTER LSHQITSRVV SAATQLRHFF LVEDLVDSLK LALLFYILTF VGAIFNGLTL481LILGVIGLFT IPLLYRQHQA QIDQYVGLVT NQLSHIKAKI RAKIPGTGAL AFAAAAVSGS541KAKAETRTRPLEQKLISEEDLAANDILDYKDDDDKV
545 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.