Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RTN1 / Reticulon 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110756-100
Catalog Number: LS-G110756
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Reticulon 1(RTN1) (NM_021136), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Reticulon-1 | NSP | Reticulon 1 | RTN1
- Origin Species
- Human
- NCBI GenBank
- NM_021136
- SwissProt
- Q16799
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 83.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAPGDPQDE LLPLAGPGSQ WLRHRGEGEN EAVTPKGATP APQAGEPSPG LGARAREAAS61REAGSGPARQ SPVAMETAST GVAGVSSAMD HTFSTTSKDG EGSCYTSLIS DICYPPQEDS121TYFTGILQKE NGHVTISESP EELGTPGPSL PDVPGIESRG LFSSDSGIEM TPAESTEVNK181ILADPLDQMK AEAYKYIDIT RPEEVKHQEQ HHPELEDKDL DFKNKDTDIS IKPEGVREPD241KPAPVEGKII KDHLLEESTF APYIDDLSEE QRRAPQITTP VKITLTEIEP SVETTTQEKT301PEKQDICLKP SPDTVPTVTV SEPEDDSPGS ITPPSSGTEP SAAESQGKGS ISEDELITAI361KEAKGLSYET AENPRPVGQL ADRPEVKARS GPPTIPSPLD HEASSAESGD SEIELVSEDP421MAAEDALPSG YVSFGHVGGP PPSPASPSIQ YSILREEREA ELDSELIIES CDASSASEES481PKREQDSPPM KPSALDAIRE ETGVRAEERA PSRRGLAEPG SFLDYPSTEP QPGPELPPGD541GALEPETPML PRKPEEDSSS NQSPAATKGP GPLGPGAPPP LLFLNKQKAI DLLYWRDIKQ601TGIVFGSFLL LLFSLTQFSV VSVVAYLALA ALSATISFRI YKSVLQAVQK TDEGHPFKAY661LELEITLSQE QIQKYTDCLQ FYVNSTLKEL RRLFLVQDLV DSLKFAVLMW LLTYVGALFN721GLTLLLMAVV SMFTLPVVYV KHQAQIDQYL GLVRTHINAV VAKIQAKIPG AKRHAETRTRPLEQKLISEEDLAANDILDYKDDDDKV
776 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.