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RTL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RTL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RTL1 antibodies →
$725.00each
Catalog Number: LS-G125356-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RTL1 (NM_001134888), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Mar1 | Retrotransposon-like protein 1 | Retrotransposon-like 1 | RTL1 | MART1 | PEG11
Origin Species
Human
NCBI GenBank
NM_001134888
SwissProt
A6NKG5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
154.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MIEPSEDSFE TMMEHKNPSS KQMESSEGSS NTTEATSGSG VRGEAGPASG PAQEKKEPPS
61GPLQEMEELP TDLLQDMEEP SSGPRKEIED PPNDLLQDLE ESCNGSHQAR GDPLSGASDR
121MKEASVNPSG AREEQEAHTD LKESGREETP QEQNQTEHST AELMAMVRSI ISLYFRMQDL
181KEQQRVAEEI LIKGINAGQL PAPKHFSGDR REFHEFIVLC QLTLQSYPRM FYNDRLRVGY
241VINHLSGLAL EWAKALLQEN SPLIGDFPAF LEAMSEVFEY RQALRVAEEA MFTIRQGGRS
301ATEYIDEFQS LVPILGWPDE VLQAHLCQGL NEEIRHYLFR VPQPDSLDSL IVLILQIEEK
361LAERRAMLRL PPEARPRNLT WIDSPAPERW MVSSWLPSEV HPDINRAHLF LLLMVRVNPY
421HSVAVQALVD SGADGNFMDE KFAQEHYVEL YEKPYPQPVQ SVDGSLIGNE PVWLYTEPLV
481CIHQNHQESI EFDIVPSPNF SVVLGIRWLR VHAPEVDWIK GRCTFHSPYC LKNCFRPPPP
541CIALERHGMS LLPGLPHPYS DLADVFNPKE ADDETSDQPS SDGSDDLSES EPSELQQAGD
601SDHSETFYEC PSTAPWEPVG ARMQERARLQ EEYWDLQDML TNRQDYIQMI PELFDQLHGA
661EWFTKLELRG TIVEESVNGH RTEDVWKAAF GLELEEMKSY QPFALSPDPI IPQNVIHFIL
721KDMLGFFVLS YGQEVLIYSM SQEEHLHHVR QVLVRFRHHN VYCSLDKSQF HRQTVEFLGF
781VVTPKGVKLN KNVMTIITGY PTPGSKLSLR NFIEFVFPYR HFVERFSIIA EPLVRQLLSS
841YQFYWGVEEQ EAFECLKRAF RKAPLLHHPK PQNPFYLETG VTGTALHASL IQIDDQTGKR
901ACCAFYSRNI SPIEVEYSQA EMKILPIRAA FMVWCRYLEN TEEPIMILLN TEDLASLNND
961RLTVLLPGHW VFFFSHFNFD VMELPEQDGG RALPPVRNLR WRRAFQRNTA ARQTLLLASR
1021GFPRDPSTES GEEENEEQDE LNEQILRQEL LAMIPIDQIL NSFLAHFSMA QIRAVILHFF
1081RGLLYWKNTL ALAAILVLLR VRQCLSLRPA PAMRVARPQP QRSLRLILDS SLIAGSSITT
1141AITQLLTQMP ALVGANTIPA QELAELFLGP GRWQRNALHS QAHRGLQFTP GFWLTLCEFF
1201GVRVTPQEGH LPALRQNRYL ELHVVGDEDV VLREALQDDL QRYRQCGLHD GLQDTSQDKQ
1261DNDVQEAPPS HTAATHPPRP RHLMDPQVLE FLGSRLLHIH SADGQLHLLS REQAARALSQ
1321FLTLIYRRAL PIPAWESQPR EQARLEELPD EDEDANLD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1358 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.