LSBio
RTKN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RTKN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RTKN2 antibodies →
$725.00each
Catalog Number: LS-G106672-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Rhotekin 2(RTKN2) (NM_145307), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA531F24.1 | Em:AC024597.2 | Rhotekin-2 | PLEKHK1 | Rhotekin 2 | RTKN2
Origin Species
Human
SwissProt
Q8IZC4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGPSLRGPA LRLAGLPTQQ DCNIQEKIDL EIRMREGIWK LLSLSTQKDQ VLHAVKNLMV
61CNARLMAYTS ELQKLEEQIA NQTGRCDVKF ESKERTACKG KIAISDIRIP LMWKDSDHFS
121NKERSRRYAI FCLFKMGANV FDTDVVNVDK TITDICFENV TIFNEAGPDF QIKVEVYSCC
181TEESSITNTP KKLAKKLKTS ISKATGKKIS SVLQEEDDEM CLLLSSAVFG VKYNLLAHTT
241LTLESAEDSF KTHNLSINGN EESSFWLPLY GNMCCRLVAQ PACMAEDAFA GFLNQQQMVE
301GLISWRRLYC VLRGGKLYCF YSPEEIEAKV EPALVVPINK ETRIRAMDKD AKKRIHNFSV
361INPVPGQAIT QIFAVDNRED LQKWMEAFWQ HFFDLSQWKH CCEELMKIEI MSPRKPPLFL
421TKEATSVYHD MSIDSPMKLE SLTDIIQKKI EETNGQFLIG QHEESLPPPW ATLFDGNHQM
481VIQKKVLYPA SEPLHDEKGK KRQAPLPPSD KLPFSLKSQS NTDQLVKDNW GKTSVSQTSS
541LDTKLSTLMH HLQKPMAAPR KLLPARRNRL SDGEHTDTKT NFEAKPVPAP RQKSIKDILD
601PRSWLQAQV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

609 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.