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RTF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RTF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RTF1 antibodies →
$504.00each
Catalog Number: LS-G113442-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RTF1 (NM_015138), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GTL7 | RTF1 | KIAA0252
Origin Species
Human
NCBI GenBank
D87440
SwissProt
Q92541

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67 kda
Protein Type
Lysate
Amino Acid Sequence
1MKKQANKTAS SGSSDKDSSA ESSAPEEGEV SDSDSNSSSS SSDSDSSSED EEFHDGYGED
61LMGDEEDRAR LEQMTEKERE QELFNRIEKR EVLKRRFEIK KKLKTAKKKE KKEKKKKQEE
121EQEKKKLTQI QESQVTSHNK ERRSKRDEKL DKKSQAMEEL KAEREKRKNR TAELLAKKQP
181LKTSEVYSDD EEEEEDDKSS EKSDRSSRTS SSDEEEEKEE IPPKSQPVSL PEELNRVRLS
241RHKLERWCHM PFFAKTVTGC FVRIGIGNHN SKPVYRVAEI TGVVETAKVY QLGGTRTNKG
301LQLRHGNDQR VFRLEFVSNQ EFTESEFMKW KEAMFSAGMQ LPTLDEINKK ELSIKEALNY
361KFNDQDIEEI VKEKERFRKA PPNYAMKKTQ LLKEKAMAED LGDQDKAKQI QDQLNELEER
421AEALDRQRTK NISAISYINQ RNREWNIVES EKALVAESHN MKNQQMDPFT RRQCKPTIVS
481NSRDPAVQAA ILAQLNAKYG SGVLPDAPKE MSKGQGKDKD LNSKSASDLS EDLFKVHDFD
541VKIDLQVPSS ESKALAITSK APPAKDGAPR RSLNLEDYKK RRGLI
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

585 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.