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RSPH10B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RSPH10B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RSPH10B antibodies →
$725.00each
Catalog Number: LS-G105212-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RSPH10B (NM_173565), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Radial spoke head 10 homolog B | RSPH10B
Origin Species
Human
SwissProt
P0C881

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVKEKKKADK KGEKSARSPS SLSDNLDFSK QDGNTTRQEM SPAGVPLLGM QLNEVKPKKD
61RQNVQQNEDA SQYEESILTK LIVESYEGEK VRGLYEGEGF AAFQGGCTYR GMFSEGLMHG
121QGTYIWADGL KYEGDFVKNV PMNHGVYTWP DGSMYEGEVV NGMRNGFGMF KCSTQPVSYI
181GHWCNGKRHG KGSIYYNQEG TCWYEGDWVQ NIKKGWGIRC YKSGNIYEGQ WEDNMRHGEG
241RMRWLTTNEE YTGRWERGIQ NGFGTHTWFL KRIRSSQYPL RNEYIGEFVN GYRHGRGKFY
301YASGAMYDGE WVSNKKHGMG RLTFKNGRVY EGAFSNDHIA GFPDLEVEFI SCLDLSSGVA
361PRLSRSAELI RKLDGSESHS VLGSSIELDL NLLLDMYPET VQPEEKKQVE YAVLRNITEL
421RRIYSFYSSL GCGHSLDNTF LMTKLHFWRF LKDCKFHHHK LTLADMDRIL SANNDIPVEE
481IHSPFTTILL RTFLNYLLHL AYHIYHEEFQ KRSPSLFLCF TKLMTENIRP NACQIKGNLF
541REQQRTLYSI SYMNKCWEIY LAYCRPSAAP PHEPTMKMRH FLWMLKDFKM INKELTAATF
601MEVIAEDNRF IYDGIDSNFE PELVFLEFFE ALLSFAFICV TDQMTKSYTN VPADDVSGNK
661HETIYTILNQ DAQNKSPSAV MSHESDAAHS DSARSSSSKL ELSPDVNKIR KSEPKIKKSV
721SHERVSKMNF KLTGKGITFF SSESKKYERP KDDREEEFNT WVNNMYVFFV NTLFHAYKRE
781EAIKEKIRAD RLRSTAQAQQ RKMEDDELEA RLNIFILREE EAKRHDYEVD ITVLKKPADV
841SSSHLILDPP KEDVTVSPSS KTITSKKKKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

870 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.