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RSHL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RSHL2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109132-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RSPH3 (NM_031924), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DJ111C20.1 | Radial spoke protein 3 | RSHL2 | RSP3 | RSPH3 | Radial spoke head 3 homolog | Radial spokehead-like 2
Origin Species
Human
NCBI GenBank
NM_031924
SwissProt
Q86UC2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTVKPAKAAS LARNLAKRRR TYLGGAAGRS QEPEVPCAAV LPGKPGDRNC PEFPPPDRTL
61GCWATDAAPA AGLCGAGSEP SIAPTSCAGN LPSRPPPLLS PLLASRNPCP WHYLHLSGSH
121NTLAPTCFKA KLHRKRGSQP PDMASALTDR TSRAPSTYTY TSRPRALPCQ RSRYRDSLTQ
181PDEEPMHYGN IMYDRRVIRG NTYALQTGPL LGRPDSLELQ RQREARKRAL ARKQAQEQLR
241PQTPEPVEGR KHVDVQTELY LEEIADRIIE VDMECQTDAF LDRPPTPLFI PAKTGKDVAT
301QILEGELFDF DLEVKPVLEV LVGKTIEQSL LEVMEEEELA NLRASQREYE ELRNSERAEV
361QRLEEQERRH REEKERRKKQ QWEIMHKHNE TSQKIAARAF AQRYLADLLP SVFGSLRDSG
421YFYDPIERDI EIGFLPWLMN EVEKTMEYSM VGRTVLDMLI REVVEKRLCM YEHGEDTHQS
481PEPEDEPGGP GAMTESLEAS EFLEQSMSQT RELLLDGGYL QRTTYDRRSS QERKFMEERE
541LLGQDEETAM RKSLGEEELS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

560 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.