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RSBN1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RSBN1L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RSBN1L antibodies →
$725.00each
Catalog Number: LS-G103714-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RSBN1L (NM_198467), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RSBN1L
Origin Species
Human
NCBI GenBank
NM_198467
SwissProt
Q6PCB5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEPPSPVHC VAAAAPTATV SEKEPFGKLQ LSSRDPPGSL SAKKVRTEEK KAPRRVNGEG
61GSGGNSRQLQ PPAAPSPQSY GSPASWSFAP LSAAPSPSSS RSSFSFSAGT AVPSSASASL
121SQPVPRKLLV PPTLLHAQPH HLLLPAAAAA ASANAKSRRP KEKREKERRR HGLGGAREAG
181GASREENGEV KPLPRDKIKD KIKERDKEKE REKKKHKVMN EIKKENGEVK ILLKSGKEKP
241KTNIEDLQIK KVKKKKKKKH KENEKRKRPK MYSKSIQTIC SGLLTDVEDQ AAKGILNDNI
301KDYVGKNLDT KNYDSKIPEN SEFPFVSLKE PRVQNNLKRL DTLEFKQLIH IEHQPNGGAS
361VIHAYSNELS HLSPMEMERF AEEFVGLVFS ENENSAAFYV MGIVHGAATY LPDFLDYFSF
421NFPNSPVLGK KDIETTTMSN FHAQVKRTYS HGTYRAGPMR QISLVGAVDE EVGDYFPEFL
481DMLEESPFLK CTLPWGTLSS LKLQSRKDSD DGPIMWVRPG EQMIPVADMP KSPFKRKRTT
541NEIKNLQYLP RTSEPREMLF EDRTRAHADH IGQGFERQTT AAVGVLKAVH CGEWPDQPRI
601TKDVICFHAE DFLEVVQRMQ LDLHEPPLSQ CVQWVDDAKL NQLRREGIRY ARIQLYDNDI
661YFIPRNVVHQ FKTVSAVCSL AWHIRLKLYH SEEDTSQNTA THETGTSSDS TSSVLGPHTD
721NMICAVSKAS LDSVFSDKLH SKYELQQIKH EPIASVRIKE EPVNVNIPEK TTALNNMDGK
781NVKAKLDHVQ FAEFKIDMDS KFENSNKDLK EELCPGNLSL VDTRQHSSAH SNQDKKDDDI
841LC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

842 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.