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RSBN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RSBN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all RSBN1 antibodies →
$725.00each
Catalog Number: LS-G111794-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RSBN1 (NM_018364), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ROSBIN | RSBN1 | RP11-324J2.1
Origin Species
Human
NCBI GenBank
NM_018364
SwissProt
Q5VWQ0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFISGRRTAD KWRAEERLQC PAGSARAALA RCADGGAVGP FKCVFVGEMA AQVGAVRVVR
61AVAAQEEPDK EGKEKPHAGV SPRGVKRQRR SSSGGSQEKR GRPSQEPPLA PPHRRRRSRQ
121HPGPLPPTNA APTVPGPVEP LLLPPPPPPS LAPAGPAVAA PLPAPSTSAL FTFSPLTVSA
181AGPKHKGHKE RHKHHHHRGP DGDPSSCGTD LKHKDKQENG ERTGGVPLIK APKRETPDEN
241GKTQRADDFV LKKIKKKKKK KHREDMRGRR LKMYNKEVQT VCAGLTRISK EILTQGQINS
301TSGLNKESFR YLKDEQLCRL NLGMQEYRVP QGVQTPFMTH QEHSIRRNFL KTGTKFSNFI
361HEEHQSNGGA LVLHAYMDEL SFLSPMEMER FSEEFLALTF SENEKNAAYY ALAIVHGAAA
421YLPDFLDYFA FNFPNTPVKM EILGKKDIET TTISNFHTQV NRTYCCGTYR AGPMRQISLV
481GAVDEEVGDY FPEFLDMLEE SPFLKMTLPW GTLSSLRLQC RSQSDDGPIM WVRPGEQMIP
541TADMPKSPFK RRRSMNEIKN LQYLPRTSEP REVLFEDRTR AHADHVGQGF DWQSTAAVGV
601LKAVQFGEWS DQPRITKDVI CFHAEDFTDV VQRLQLDLHE PPVSQCVQWV DEAKLNQMRR
661EGIRYARIQL CDNDIYFIPR NVIHQFKTVS AVCSLAWHIR LKQYHPVVEA TQNTESNSNM
721DCGLTGKREL EVDSQCVRIK TESEEACTEI QLLTTASSSF PPASELNLQQ DQKTQPIPVL
781KVESRLDSDQ QHNLQEHSTT SV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

802 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.