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RREB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

RREB1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G122471-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RREB1 (NM_001003699), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNA-binding protein | FINB | Hindsight homolog | RREB1 | Zep-1 | HNT | LZ321 | RREB-1
Origin Species
Human
NCBI GenBank
NM_001003699
SwissProt
Q92766

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
187.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTSSSPAGLE GSDLSSINTM MSAVMSVGKV TENGGSPQGI KSPSKPPGPN RIGRRNQETK
61EEKSSYNCPL CEKICTTQHQ LTMHIRQHNT DTGGADHSCS ICGKSLSSAS SLDRHMLVHS
121GERPYKCTVC GQSFTTNGNM HRHMKIHEKD PNSATATAPP SPLKRRRLSS KRKLSHDAES
181EREDPAPAKK MVEDGQSGDL EKKADEVFHC PVCFKEFVCK YGLETHMETH SDNPLRCDIC
241CVTFRTHRGL LRHNALVHKQ LPRDAMGRPF IQNNPSIPAG FHDLGFTDFS CRKFPRISQA
301WCETNLRRCI SEQHRFVCDT CDKAFPMLCS LALHKQTHVA ADQGQEKPQA TPLPGDALDQ
361KGFLALLGLQ HTKDVRPAPA EEPLPDDNQA IQLQTLKCQL PQDPGCTNLL SLSPFEAASL
421GGSLTVLPAT KDSIKHLSLQ PFQKGFIIQP DSSIVVKPIS GESAIELADI QQILKMAASA
481PPQISLPPFS KAPAAPLQAI FKHMPPLKPK PLVTPRTVVA TSTPPPLINA QQASPGCISP
541SLPPPPLKLL KGSVEAASNA HLLQSKSGTQ PHAATRLSLQ QPRAELPGQP EMKTQLEQDS
601IIEALLPLSM EAKIKQEITE GELKAFMTAP GGKKTPAMRK VLYPCRFCNQ VFAFSGVLRA
661HVRSHLGISP YQCNICDYIA ADKAALIRHL RTHSGERPYI CKICHYPFTV KANCERHLRK
721KHLKATRKDI EKNIEYVSSS AAELVDAFCA PDTVCRLCGE DLKHYRALRI HMRTHCGRGL
781GGGHKGRKPF ECKECSAAFA AKRNCIHHIL KQHLHVPEQD IESYVLAADG LGPAEAPAAE
841ASGRGEDSGC AALGDCKPLT AFLEPQNGFL HRGPTQPPPP HVSIKLEPAS SFAVDFNEPL
901DFSQKGLALV QVKQENISFL SPSSLVPYDC SMEPIDLSIP KNFRKGDKDL ATPSEAKKPE
961EEAGSSEQPS PCPAPGPSLP VTLGPSGILE SPMAPAPAAT PEPPAQPLQG PVQLAVPIYS
1021SALVSSPPLV GSSALLSGTA LLRPLRPKPP LLLPKPPVTE ELPPLASIAQ IISSVSSAPT
1081LLKTKVADPG PASTGSNTTA SDSLGGSVPK AATTATPAAT TSPKESSEPP APASSPEAAS
1141PTEQGPAGTS KKRGRKRGMR SRPRANSGGV DLDSSGEFAS IEKMLATTDT NKFSPFLQTA
1201EDNTQDEVAG APADHHGPSD EEQGSPPEDK LLRAKRNSYT NCLQKITCPH CPRVFPWASS
1261LQRHMLTHTG QKPFPCQKCD AFFSTKSNCE RHQLRKHGVT TCSLRRNGLI PQSKESDVGS
1321HDSTDSQSDA ETAAAAGEVL DLTSRDREQP SEGATELRQV AGDAPVEQAT AETASPVHRE
1381EHGRGESHEP EEEHGTEEST GDADGAEEDA SSNQSLDLDF ATKLMDFKLA EGDGEAGAGG
1441AASQEQKLAC DTCGKSFKFL GTLSRHRKAH GRQEPKDEKG DGASTAEEGP QPAPEQEEKP
1501PETPAEVVES APGAGEAPAE KLAEETEGPS DGESAAEKRS SEKSDDDKKP KTDSPKSVAS
1561KADKRKKVCS VCNKRFWSLQ DLTRHMRSHT GERPYKCQTC ERTFTLKHSL VRHQRIHQKA
1621RHAKHHGKDS DKEERGEEDS ENESTHSGNN AVSENEAELA PNASNHMAVT RSRKEGLASA
1681TKDCSHREEK VTAGWPSEPG QGDLNPESPA ALGQDLLEPR SKRPAHPILA TADGASQLVG
1741ME
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1742 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.