Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RRBP1 / hES Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116547-100
Catalog Number: LS-G116547
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RRBP1 (NM_004587), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ES/130 | ES/130-related protein | KIAA1398 | Ribosome binding protein 1 | Ribosome receptor protein | Ribosome-binding protein 1 | RRBP1 | RRP | ES130 | HES
- Origin Species
- Human
- NCBI GenBank
- NM_004587
- SwissProt
- Q9P2E9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 108.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDIYDTQTLG VVVFGGFMVV SAIGIFLVST FSMKETSYEE ALANQRKEMA KTHHQKVEKK61KKEKTVEKKG KTKKKEEKPN GKIPDHDPAP NVTVLLREPV RAPAVAVAPT PVQPPIIVAP121VATVPAMPQE KLASSPKDKK KKEKKVAKVE PAVSSVVNSI QVLTSKAAIL ETAPKEGRNT181DVAQSPEAPK QEAPAKKKSG SKKKGPPDAD GPLYLPYKTL VSTVGSMVFN EGEAQRLIEI241LSEKAGIIQD TWHKATQKGD PVAILKRQLE EKEKLLATEQ EDAAVAKSKL RELNKEMAAE301KAKAAAGEAK VKKQLVAREQ EITAVQARMQ ASYREHVKEV QQLQGKIRTL QEQLENGPNT361QLARLQQENS ILRDALNQAT SQVESKQNAE LAKLRQELSK VSKELVEKSE AVRQDEQQRK421ALEAKAAAFE KQVLQLQASH RESEEALQKR LDEVSRELCH TQSSHASLRA DAEKAQEQQQ481QMAELHSKLQ SSEAEVRSKC EELSGLHGQL QEARAENSQL TERIRSIEAL LEAGQARDAQ541DVQASQAEAD QQQTRLKELE SQVSGLEKEA IELREAVEQQ KVKNNDLREK NWKAMEALAT601AEQACKEKLH SLTQAKEESE KQLCLIEAQT MEALLALLPE LSVLAQQNYT EWLQDLKEKG661PTLLKHPPAP AEPSSDLASK LREAEETQST LQAECDQYRS ILAETEGMLR DLQKSVEEEE721QVWRAKVGAA EEELQKSRVT VKHLEEIVEK LKGELESSDQ VREHTSHLEA ELEKHMAAAS781AECQNYAKEV AGLRQLLLES QSQLDAAKSE AQKQSDELAL VRQQLSEMKS HVEDGDIAGA841PASSPEAPPA EQDPVQLKTQ LEWTEAILED EQTQRQKLMA EFEEAQTSAC RLQEELEKLR901TAGPLESSET EEASQLKERL EKEKKLTSDL GRAATRLQEL LKTTQEQLAR EKDTVKKLQE961QLEKAEDGSS SKEGTSVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
977 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.