Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
RRAGC / RAGC Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110422-100
Catalog Number: LS-G110422
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RRAGC (NM_022157), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- GTPase-interacting protein 2 | GTR2 | RAGC | RRAGC | Rag C | Rag C protein | Ras-related GTP binding C | TIB929
- Origin Species
- Human
- NCBI GenBank
- NM_022157
- SwissProt
- Q9HB90
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 44 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSLQYGAEET PLAGSYGAAD SFPKDFGYGV EEEEEEAAAA GGGVGAGAGG GCGPGGADSS61KPRILLMGLR RSGKSSIQKV VFHKMSPNET LFLESTNKIY KDDISNSSFV NFQIWDFPGQ121MDFFDPTFDY EMIFRGTGAL IYVIDAQDDY MEALTRLHIT VSKAYKVNPD MNFEVFIHKV181DGLSDDHKIE TQRDIHQRAN DDLADAGLEK LHLSFYLTSI YDHSIFEAFS KVVQKLIPQL241PTLENLLNIF ISNSGIEKAF LFDVVSKIYI ATDSSPVDMQ SYELCCDMID VVIDVSCIYG301LKEDGSGSAY DKESMAIIKL NNTTVLYLKE VTKFLALVCI LREESFERKG LIDYNFHCFR361KAIHEVFEVG VTSHRSCGHQ TSASSLKALT HNGTPRNAITRTRPLEQKLISEEDLAANDILDYKDDDDKV
399 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.